Biochemistry analyzer for the modeling and control of mammalian cell culture processes

用于哺乳动物细胞培养过程建模和控制的生化分析仪

• 批准号: 345307-2007
• 负责人: Henry, Olivier
• 金额: $ 3.73万
• 依托单位: École Polytechnique de Montréal
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2006
• 资助国家: 加拿大
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=325638
• 关键词: Biochemistry; analyzer; modeling; control; mammalian

This research proposal aims at the acquisition of a biochemistry analyzer capable of providing rapid and simultaneous measurements of key metabolites in mammalian cell cultures. The requested equipment is intended primarily to support research focused on improving the development and control of mammalian cell culture processes. Mammalian cells are increasingly being used for the manufacture of high-value biopharmaceuticals products such as monoclonal antibodies, viral vaccines and hormones. The growing demand for high volume products is driving the need to improve the productivity and cost efficiency of cell culture processes. Despite progress in the field, significant challenges remain to achieve desired product quality and increase the yields. The proposed research aims at understanding and characterising the cellular metabolism and physiology, as a basis for the development and control of cell culture processes. The methodology relies heavily on the ability to sensitively and rapidly measure important bioprocess variables. By addressing unmet quantification needs, the analyzer will be crucial for the realization of the research project. This research is expected to bring a significant contribution to the field of animal cell technology and have an impact on the development of bioprocesses at all scales of operation, from the small amounts required for preclinical studies through to bulk manufacture. Advances in this area of research will contribute to help eliminating the bottleneck in the development of biotherapeutics, thus impacting positively on the health of Canadians and the Canadian biopharmaceutical industry. The instrument was also selected for its potential to support on-going and new emerging mammalian cell culture research activities at Ecole Polytechnique in promising areas such as tissue engineering and biomedecine. Its availability will also positively impact the training of students by providing them with hands-on experience on state-of-the-art equipment.

这项研究建议旨在获得一种生化分析仪，能够快速和同时测量哺乳动物细胞培养中的关键代谢物。所要求的设备主要是为了支持侧重于改进哺乳动物细胞培养过程的开发和控制的研究。哺乳动物细胞越来越多地被用于制造高价值的生物制药产品，如单抗、病毒疫苗和激素。对大批量产品日益增长的需求推动了提高细胞培养过程的生产率和成本效益的需求。尽管在这一领域取得了进展，但在实现预期的产品质量和提高产量方面仍然面临重大挑战。这项拟议的研究旨在了解和表征细胞的新陈代谢和生理学，作为发展和控制细胞培养过程的基础。该方法在很大程度上依赖于敏感而快速地测量重要生物过程变量的能力。通过解决未得到满足的量化需求，分析器将是实现研究项目的关键。这项研究有望为动物细胞技术领域带来重大贡献，并对所有规模的生物过程的发展产生影响，从临床前研究所需的少量到大规模生产。这一研究领域的进展将有助于消除生物疗法发展的瓶颈，从而对加拿大人的健康和加拿大生物制药业产生积极影响。该仪器之所以被选中，也是因为它有潜力支持Ecole Polytech在组织工程和生物医学等前景广阔的领域正在进行的和新出现的哺乳动物细胞培养研究活动。它的推出还将通过为学生提供最先进设备的实践体验，对学生的培训产生积极影响。