Nanoparticle Tracking Analyzer (NTA) for the Center for Live Cell Genomics

用于活细胞基因组学中心的纳米颗粒跟踪分析仪 (NTA)

• 批准号: 10817569
• 负责人: DAVID H HAUSSLER
• 金额: $ 20.14万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA CRUZ
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-15 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10817569
• 关键词: 3-Dimensional; Acceleration; Address; Biological; Biopsy; Brain; Buffers; Calibration; Cell Culture Techniques; Cells; Cerebral cortex; Cerebrum; Charge; Communication; Complex; Computer software; Computers; Data; Development; Devices; Disease model; Distant; Ecosystem; Funding; Genes; Genomics; Goals; Growth; Hallmark Cell; Health; Human; Individual; Internet; Internet of Things; Label; Link; Maintenance; Malignant Childhood Neoplasm; Malignant Neoplasms; Measurement; Measures; Metabolic; Metabolism; Methodology; Methods; Modeling; Molecular; Neurodevelopmental Disorder; Operative Surgical Procedures; Organoids; Outcome; Pathway interactions; Performance; Population; Principal Investigator; Reproducibility; Research; Research Personnel; Scientist; Side; Surface; System; Techniques; Technology; Time; Tissue Model; Tissues; Training; Vendor; biological systems; cell type; design; exosome; experimental study; extracellular vesicles; flexibility; innovation; instrument; multimodality; nanoparticle; nanoscale; neurodevelopment; new technology; open source; operation; programs; remote control; sensor; single molecule; three dimensional cell culture; tissue culture; tissue/cell culture

Principal Investigator/Program Director: Haussler, David Project Summary The focus of our CEGS Center for Live Cell Genomics is to build new methodology and capacity for large-scale, long-term, inexpensive, modular, customizable, shared, Internet-of-Things- controlled, reproducible live cell culture and tissue-based experimental genomics disease models. Tissue models include traditional cell culture as well as organoid and primary tissue explants obtained from surgery or biopsy. A particular focus is the integration of organoid factories that support tissue growth and maintenance with external and on-chip electro- optofluidic analytical modules to become part of an ecosystem that is modeled after open- source software. This system will use commodity sensors, cameras, and computers linked in platforms that are flexibly designed using simple, widely available techniques potentially in order to stimulate rapid innovation in experimental platforms for tissue culture. This novel technology will allow us to address major scientific issues in neurodevelopment and pediatric cancer. These include questions about what genes contribute to human brain development, or what specific molecular pathways are disrupted in individual pediatric cancer cases. Over the first year of our project, it has become clear that in order to advance our research agenda on both the scientific and technological sides we require an established, multi-modal nanoparticle analysis method. Specifically, we are facing two challenges. We need to be able to assess the nanoscale extracellular vesicles (EVs), specifically exosomes, that are being produced in our organoid factories as a measure of organoid cell types, metabolism, and health. As these measurements need to be carried out frequently over long periods of time, a fast and relatively easy-to-use technique is required. Secondly, in order to develop optofluidic devices that can carry out measurements on individual EVs and exosomes and their molecular content, we need to be able to establish ground truth references against which the performance of these devices can be compared. To this end, we are requesting supplemental funding to acquire a Nanoparticle Tracking Analyzer (NTA) instrument that will provide these capabilities throughout our project. An NTA is a unique instrument that provides multi-modal analysis on populations of biological and inorganic nanoparticles. Pertinent capabilities include buffer calibration, measurement of EV concentration and size distribution, surface charge measurements, assessment of labeling efficiencies, and more. As such, an NTA is uniquely suited for our requirements and will dramatically accelerate the progress of our research as well as expand its outcomes. 1

首席研究员/项目总监：Haussler, David 项目概要 我们的 CEGS 活细胞基因组中心的重点是建立新的方法和能力 大规模、长期、廉价、模块化、可定制、共享、物联网控制、可复制 活细胞培养和基于组织的实验基因组学疾病模型。组织模型包括传统细胞 培养物以及从手术或活检获得的类器官和原代组织外植体。一个特别的重点是 支持组织生长和维护的类器官工厂与外部和片上的集成 光电流控分析模块将成为开源生态系统的一部分 软件。该系统将使用连接在灵活平台上的商品传感器、摄像头和计算机。 使用简单、广泛可用的技术进行设计，可能是为了刺激快速创新 组织培养实验平台。这项新技术将使我们能够解决重大科学问题 在神经发育和小儿癌症方面。这些问题包括哪些基因对人类有贡献 大脑发育，或者在个别儿科癌症病例中哪些特定的分子途径被破坏。 在我们项目的第一年，很明显，为了推进我们的研究议程 科学和技术方面，我们需要一种成熟的、多模式的纳米粒子分析方法。 具体来说，我们面临两个挑战。我们需要能够评估纳米级细胞外囊泡 （EV），特别是外泌体，在我们的类器官工厂中生产，作为类器官细胞的衡量标准 类型、新陈代谢和健康。由于这些测量需要在很长一段时间内频繁进行 随着时间的推移，需要一种快速且相对易于使用的技术。其次，为了开发光流控器件 可以对单个 EV 和外泌体及其分子含量进行测量，我们需要 能够建立可以比较这些设备性能的地面实况参考。到 为此，我们请求补充资金来购买纳米粒子跟踪分析仪（NTA） 仪器将在我们的整个项目中提供这些功能。 NTA 是一种独特的工具， 提供对生物和无机纳米粒子群体的多模式分析。相关能力 包括缓冲液校准、EV浓度和尺寸分布的测量、表面电荷 测量、标签效率评估等。因此，NTA 特别适合我们 要求，并将大大加快我们的研究进展并扩大其成果。 1

项目成果

Dual optofluidic distributed feedback dye lasers for multiplexed biosensing applications.

• DOI: 10.1038/s41598-023-42671-4
• 发表时间: 2023-10-06
• 期刊: Scientific reports
• 影响因子: 4.6

All-in-One Optofluidic Chip for Molecular Biosensing Assays.

• DOI: 10.3390/bios12070501
• 发表时间: 2022-07-09
• 期刊: Biosensors

Model-Agnostic Neural Mean Field With The Refractory SoftPlus Transfer Function.

具有 Refractory SoftPlus 传递函数的模型无关神经平均场。

• DOI: 10.1101/2024.02.05.579047
• 发表时间: 2024
• 期刊: bioRxiv : the preprint server for biology
• 作者: Spaeth,Alex;Haussler,David;Teodorescu,Mircea
• 通讯作者: Teodorescu,Mircea

Modulation of neuronal activity in cortical organoids with bioelectronic delivery of ions and neurotransmitters.

通过离子和神经递质的生物电子传递来调节皮质类器官中的神经元活动。

• DOI: 10.1101/2023.06.10.544416
• 发表时间: 2023
• 期刊: bioRxiv : the preprint server for biology
• 作者: Park,Yunjeong;Hernandez,Sebastian;Hernandez,CristianO;Schweiger,HunterE;Li,Houpu;Voitiuk,Kateryna;Dechiraju,Harika;Hawthorne,Nico;Muzzy,ElanaM;Selberg,JohnA;Sullivan,FrederikaN;Urcuyo,Roberto;Salama,SofieR;Aslankoohi,Elham
• 通讯作者: Aslankoohi,Elham

Cerebral Organoids as an Experimental Platform for Human Neurogenomics.

• DOI: 10.3390/cells11182803
• 发表时间: 2022-09-08
• 期刊: CELLS
• 影响因子: 6
• 作者: Nowakowski, Tomasz J.;Salama, Sofie R.
• 通讯作者: Salama, Sofie R.