Nanoparticle Tracking Analyzer (NTA) for the Center for Live Cell Genomics

用于活细胞基因组学中心的纳米颗粒跟踪分析仪 (NTA)

• 批准号: 10817569
• 负责人: DAVID H HAUSSLER
• 金额: $ 20.14万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA CRUZ
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-15 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10817569
• 关键词: 3-Dimensional; Acceleration; Address; Biological; Biopsy; Brain; Buffers; Calibration; Cell Culture Techniques; Cells; Cerebral cortex; Cerebrum; Charge; Communication; Complex; Computer software; Computers; Data; Development; Devices; Disease model; Distant; Ecosystem; Funding; Genes; Genomics; Goals; Growth; Hallmark Cell; Health; Human; Individual; Internet; Internet of Things; Label; Link; Maintenance; Malignant Childhood Neoplasm; Malignant Neoplasms; Measurement; Measures; Metabolic; Metabolism; Methodology; Methods; Modeling; Molecular; Neurodevelopmental Disorder; Operative Surgical Procedures; Organoids; Outcome; Pathway interactions; Performance; Population; Principal Investigator; Reproducibility; Research; Research Personnel; Scientist; Side; Surface; System; Techniques; Technology; Time; Tissue Model; Tissues; Training; Vendor; biological systems; cell type; design; exosome; experimental study; extracellular vesicles; flexibility; innovation; instrument; multimodality; nanoparticle; nanoscale; neurodevelopment; new technology; open source; operation; programs; remote control; sensor; single molecule; three dimensional cell culture; tissue culture; tissue/cell culture

Principal Investigator/Program Director: Haussler, David Project Summary The focus of our CEGS Center for Live Cell Genomics is to build new methodology and capacity for large-scale, long-term, inexpensive, modular, customizable, shared, Internet-of-Things- controlled, reproducible live cell culture and tissue-based experimental genomics disease models. Tissue models include traditional cell culture as well as organoid and primary tissue explants obtained from surgery or biopsy. A particular focus is the integration of organoid factories that support tissue growth and maintenance with external and on-chip electro- optofluidic analytical modules to become part of an ecosystem that is modeled after open- source software. This system will use commodity sensors, cameras, and computers linked in platforms that are flexibly designed using simple, widely available techniques potentially in order to stimulate rapid innovation in experimental platforms for tissue culture. This novel technology will allow us to address major scientific issues in neurodevelopment and pediatric cancer. These include questions about what genes contribute to human brain development, or what specific molecular pathways are disrupted in individual pediatric cancer cases. Over the first year of our project, it has become clear that in order to advance our research agenda on both the scientific and technological sides we require an established, multi-modal nanoparticle analysis method. Specifically, we are facing two challenges. We need to be able to assess the nanoscale extracellular vesicles (EVs), specifically exosomes, that are being produced in our organoid factories as a measure of organoid cell types, metabolism, and health. As these measurements need to be carried out frequently over long periods of time, a fast and relatively easy-to-use technique is required. Secondly, in order to develop optofluidic devices that can carry out measurements on individual EVs and exosomes and their molecular content, we need to be able to establish ground truth references against which the performance of these devices can be compared. To this end, we are requesting supplemental funding to acquire a Nanoparticle Tracking Analyzer (NTA) instrument that will provide these capabilities throughout our project. An NTA is a unique instrument that provides multi-modal analysis on populations of biological and inorganic nanoparticles. Pertinent capabilities include buffer calibration, measurement of EV concentration and size distribution, surface charge measurements, assessment of labeling efficiencies, and more. As such, an NTA is uniquely suited for our requirements and will dramatically accelerate the progress of our research as well as expand its outcomes. 1

首席研究员/项目主管：Haussler， David

项目成果

Dual optofluidic distributed feedback dye lasers for multiplexed biosensing applications.

• DOI: 10.1038/s41598-023-42671-4
• 发表时间: 2023-10-06
• 期刊: Scientific reports
• 影响因子: 4.6

All-in-One Optofluidic Chip for Molecular Biosensing Assays.

• DOI: 10.3390/bios12070501
• 发表时间: 2022-07-09
• 期刊: Biosensors

Model-Agnostic Neural Mean Field With The Refractory SoftPlus Transfer Function.

具有 Refractory SoftPlus 传递函数的模型无关神经平均场。

• DOI: 10.1101/2024.02.05.579047
• 发表时间: 2024
• 期刊: bioRxiv : the preprint server for biology
• 作者: Spaeth,Alex;Haussler,David;Teodorescu,Mircea
• 通讯作者: Teodorescu,Mircea

Modulation of neuronal activity in cortical organoids with bioelectronic delivery of ions and neurotransmitters.

通过离子和神经递质的生物电子传递来调节皮质类器官中的神经元活动。

• DOI: 10.1101/2023.06.10.544416
• 发表时间: 2023
• 期刊: bioRxiv : the preprint server for biology
• 作者: Park,Yunjeong;Hernandez,Sebastian;Hernandez,CristianO;Schweiger,HunterE;Li,Houpu;Voitiuk,Kateryna;Dechiraju,Harika;Hawthorne,Nico;Muzzy,ElanaM;Selberg,JohnA;Sullivan,FrederikaN;Urcuyo,Roberto;Salama,SofieR;Aslankoohi,Elham
• 通讯作者: Aslankoohi,Elham

Cerebral Organoids as an Experimental Platform for Human Neurogenomics.

• DOI: 10.3390/cells11182803
• 发表时间: 2022-09-08
• 期刊: CELLS
• 影响因子: 6
• 作者: Nowakowski, Tomasz J.;Salama, Sofie R.
• 通讯作者: Salama, Sofie R.