High pressure liquid chromatography system for analyzing photosynthetic algae and bacteria in surface waters and aquatic sediments

用于分析地表水和水生沉积物中光合藻类和细菌的高压液相色谱系统

• 批准号: 344594-2007
• 负责人: Hall, Roland
• 金额: $ 5.61万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2006
• 资助国家: 加拿大
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=325813
• 关键词: pressure; liquid; chromatography; system; analyzing

This proposal requests a High Pressure Liquid Chromatography (HPLC) system for analyzing pigments from photosynthetic algae, bacteria and higher plants in samples of water, benthos (substrates on the bottom of lakes and streams), biota and sediment.  Specifically, we request an Agilent 1200 Series HPLC system with autoanalyzer and other specifications that allow rapid quantification and identification of pigments in large numbers of samples (20 samples/day) to support research and training programs of four professors dedicated to understanding how natural aquatic systems respond to, and recover from, human disturbances and natural phenomena.  The requested equipment will allow significant improvements in terms of quantity and quality of data that can be produced with existing and future research funds by allowing research programs to quantify responses of algae and photosynthetic bacteria via machine-aided measurements of pigment molecules, rather than time-consuming, costly analyses by humans using microscopes.  The equipment will provide important information to determine the impacts of climate variability, river regulation and consumptive water use on aquatic systems in important floodplain environments (e.g., Mackenzie Drainage Basin); to understand linkages between meteorological conditions and primary producers in Lake Bosumtwi (Ghana) as required to interpret 1 million year long records of climate change from equatorial west Africa; to develop innovative new methods to monitor water quality using benthic algae to improve management of surface waters on regional to national scales; to improve our understanding of factors promoting excessive growth of nuisance algae and production of toxins by algae; and to improve understanding of how free-floating algae (phytoplankton) in lakes adapt to stresses such as UV radiation. The proposed HPLC system will significantly enhance opportunities for training highly qualified personnel in state-of-the-art techniques in aquatic ecology, hydro-ecology and paleoecology.  Use of HPLC systems by private and government sectors is increasing, thus, training our students on the proposed equipment will enhance their employment opportunities.

本提案要求建立高压液相色谱仪系统，用于分析水、底栖动物(湖泊和溪流底部的底物)、生物群和沉积物样品中来自光合作用藻类、细菌和高等植物的色素。具体地说，我们要求安捷伦1200系列高效液相色谱系统和自动分析仪以及其他规格，允许快速定量和鉴定大量样本(每天20个样本)中的色素，以支持四位教授的研究和培训计划，这些教授致力于了解自然水生系统如何响应和恢复，人类干扰和自然现象。所要求的设备将使现有和未来研究资金能够产生的数据的数量和质量得到显著改善，方法是允许研究程序通过机器辅助测量色素分子来量化藻类和光合细菌的反应，而不是由人类使用显微镜进行耗时、昂贵的分析。这些设备将提供重要信息，以确定气候变异性、河流调节和消耗性用水对重要泛滥平原环境(如麦肯齐流域)水生系统的影响；了解博苏姆特维湖(加纳)气象条件与初级生产者之间的联系，以解释来自赤道西非的100万年气候变化记录；开发利用底栖藻类监测水质的创新新方法，以改善区域和国家层面的地表水管理；提高我们对促使滋扰藻类过度生长和藻类产生毒素的因素的认识；以及提高对湖泊中自由漂浮的藻类(浮游植物)如何适应紫外线辐射等压力的认识。拟议的高效液相系统将大大增加培训高素质水生态、水生态和古生态技术人员的机会。私营部门和政府部门对高效液相系统的使用正在增加，因此，培训学生使用拟议的设备将增加他们的就业机会。