Gas chromatograph

我们需要气相色谱仪（GC），这是一种复杂的分析工具，用于测量样品提取物中的有机化合物。我们建议使用该仪器来测量在海洋食物网、水产养殖和识别海洋生物（包括有毒生物）中至关重要的化合物。我们将使用我们正在开发的一种新的脂质分析程序来确定脂肪酸，包括必需的omega-3脂肪酸、固醇（包括胆固醇）和脂质类别。该仪器将取代我们16年的nserc资助的气相色谱已经损坏无法修复。所选择的模型及其配置将使我们能够扩展到两个新的重要研究领域：氨基酸和脂质氧化产物。人们对膳食脂类和氨基酸在海洋生态系统和水产养殖中的重要性以及将海洋脂类用作生物标志物的重要性越来越感兴趣。海洋脂类包括几种在结构和功能上有相当大差异的脂类。结构上的差异允许它们作为生物标记物使用。在生物标志物方法中，某些特征化合物被用来整合定义生态系统状态的生物、物理和化学参数。使用这些化合物，我们可以量化必需营养素和有害化合物的输入，我们可以追踪它们的途径。GC将支持几个项目，其中我们的任务是确定脂质化合物和氨基酸，这些化合物和氨基酸对动物生长至关重要，或者可以用作海洋环境中发生的过程的标记。我们正在研究的一些非常重要的标志物或特征化合物可能会被用作有害藻华的早期预警。我们还提出了开发有毒脂质氧化产物的方法。这类化合物通常结构相当复杂，含量很低，因此需要使用精密的分析设备进行测定。考虑到地面渔业持续存在的问题、对新渔业物种的兴趣以及该地区水产养殖水平的提高，对纽芬兰食物网中的脂质、脂质氧化产物和氨基酸进行测量是及时的。该仪器将提供对加拿大水产养殖中鳍鱼和双壳类鱼类及幼虫的营养管理至关重要的数据。

We are requesting a gas chromatograph (GC), which is a sophisticated analytical tool used to measure organic compounds in sample extracts. We propose to use this instrument to measure compounds that are critical in marine food webs, aquaculture, and in identifying marine organisms including those that are toxic. We will determine fatty acids including essential omega-3 fatty acids, sterols including cholesterol, and lipid classes using a novel lipid profiling procedure we are developing. This instrument will replace our 16 year old NSERC-funded GC that has broken down beyond repair. The model chosen and its configuration will be such as to allow us to also expand into two new, important areas of research: amino acids and lipid oxidation products. There is considerable and growing interest in the importance of dietary lipids and amino acids in marine ecosystems and aquaculture, as well as in the use of marine lipids as biomarkers. Marine lipids comprise several lipid classes with considerable differences both in structure and function. The differences in structure permit their use as biomarkers. In the biomarker approach, certain signature compounds are used to integrate biological, physical and chemical parameters that define the status of an ecosystem. Using these compounds we can quantify inputs of essential nutrients and of deleterious compounds and we can trace their pathways. The GC will support several projects wherein our task is to determine lipid compounds and amino acids that are either critical to animal growth or that can be used as markers of processes occurring in the marine environment. Some very important marker or signature compounds we are working on are ones that may be used as an early warning of harmful algal blooms. We also propose to develop methods for toxic lipid oxidation products. Such compounds are often structurally quite complex and are present at very low levels, hence the use of sophisticated analytical equipment is required for their determination. Measurement of lipids, lipid oxidation products and amino acids in Newfoundland food webs is timely given the continuing problems with the ground fishery, interest in new fishery species, and the increasing level of aquaculture in this area. This instrument will provide data that are critical to the nutritional management of finfish and bivalve broodstock and larvae in Canadian aquaculture.