2-D mechano-optical system for the quantitative analysis of biomaterials and live cell systems

用于生物材料和活细胞系统定量分析的二维机械光学系统

• 批准号: 345134-2007
• 负责人: Gosline, John
• 金额: $ 5.13万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2006
• 资助国家: 加拿大
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=326043
• 关键词: mechano; optical; system; quantitative; analysis

This equipment grant will allow the applicants to create a microscope-based test system that can combine bi-axial mechanical measurements with quantitative, 2-dimensional birefringence measurements.  The system will be constructed on an inverted microscope, to provide working space for the mechanical test system.  The CRI PolScope birefringence imaging system will allow us to measure the magnitude and orientation of birefringence at all pixels in a digital image of the mechanical test specimen.  This facility makes it possible to document the role of tissue microstructure to mechanical properties.  In addition, the microscope system will have facilities for fluorescence microscopy so that antibody stains or GFP constructs can be used to identify specific proteins in tissues and living cells.  Having the birefringence and fluorescence imaging systems on the same microscope will allow the co-localization of ordered structures and specific proteins at the resolution of the optical system.  We will use this system to investigate the structure-property relationships of collagen and elastin based connective tissues, intermediate filament based structural systems and myofilament systems.

该设备拨款将允许申请人创建一个基于显微镜的测试系统，该系统可以将双轴机械测量与定量的二维双折射测量相结合。  该系统将构建在倒置显微镜上，为机械测试系统提供工作空间。  CRI PolScope 双折射成像系统将使我们能够测量机械测试样本数字图像中所有像素的双折射的幅度和方向。  该设施使得记录组织微观结构对机械性能的作用成为可能。  此外，显微镜系统还将配备荧光显微镜设施，以便可以使用抗体染色剂或 GFP 构建体来识别组织和活细胞中的特定蛋白质。  在同一台显微镜上安装双折射和荧光成像系统将允许在光学系统的分辨率下对有序结构和特定蛋白质进行共定位。  我们将使用该系统来研究基于胶原蛋白和弹性蛋白的结缔组织、基于中间丝的结构系统和肌丝系统的结构-性能关系。