Biosafety Hood and Ultra low temp freezer

对微生物在机会性人类感染中所起作用的认识提高，导致在对这些微生物及其去污进行研究时更加警惕。 布鲁克大学的几个实验室一直在使用过时的，现在被禁止的，培养和操作几种微生物的台式程序。在这项提案中，三个实验室（Bidochka、Hunter和Carlone）的研究包括病原真菌、西尼罗河病毒、大肠杆菌基因克隆和细胞培养的研究。 为了符合实验室生物安全指南，加拿大公共卫生署（2004年）以及实践良好的研究方案，我们需要一个生物安全罩，至少符合2级标准。我们还需要一台超低温冷冻机，以帮助保存真菌、病毒和细胞培养物的储备培养物。

Increased awareness of the role that microorganisms play in opportunistic human infections has led to greater vigilance when performing research with these microorganisms and their decontamination.  Several laboratories at Brock University have been utilizing outdated and, now prohibited, bench-top procedures in the culture and manipulation of several microorganisms. In this proposal, research from three laboratories (Bidochka, Hunter and Carlone) include studies on pathogenic fungi, West Nile virus, gene cloning with Escherichia coli and cell culture.  In order to be in compliance with Laboratory Biosafety Guidelines, Public Health Agency of Canada (2004) as well as to practise good research protocol we require a Biosafety Hood that complies with, at least, Level 2 standards. We also request an ultra-low temperature freezer to assist in maintaining stock cultures of fungi, viruses and cell cultures.