Biosafety Hood and Ultra low temp freezer

生物安全罩和超低温冰箱

• 批准号: 344784-2007
• 负责人: Bidochka, Michael
• 金额: $ 1.49万
• 依托单位: Brock University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2006
• 资助国家: 加拿大
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=326067
• 关键词: Biosafety; Hood; Ultra; low; temp

Increased awareness of the role that microorganisms play in opportunistic human infections has led to greater vigilance when performing research with these microorganisms and their decontamination.  Several laboratories at Brock University have been utilizing outdated and, now prohibited, bench-top procedures in the culture and manipulation of several microorganisms. In this proposal, research from three laboratories (Bidochka, Hunter and Carlone) include studies on pathogenic fungi, West Nile virus, gene cloning with Escherichia coli and cell culture.  In order to be in compliance with Laboratory Biosafety Guidelines, Public Health Agency of Canada (2004) as well as to practise good research protocol we require a Biosafety Hood that complies with, at least, Level 2 standards. We also request an ultra-low temperature freezer to assist in maintaining stock cultures of fungi, viruses and cell cultures.

随着人们对微生物在人类机会性感染中所起作用的认识的提高，人们在研究这些微生物及其去污时也更加警惕。布罗克大学的几个实验室一直在利用过时的、现在已被禁止的台式程序培养和操纵几种微生物。在本提案中，三个实验室（Bidochka、Hunter和Carlone）的研究包括病原真菌、西尼罗河病毒、大肠杆菌基因克隆和细胞培养研究。为了符合加拿大公共卫生署实验室生物安全指南（2004年）以及实施良好的研究方案，我们需要至少符合2级标准的生物安全罩。我们还需要一台超低温冷冻机，以帮助保存真菌、病毒和细胞培养物的储备培养物。