Automated microbiology growth curve analysis system

自动化微生物生长曲线分析系统

• 批准号: 345734-2007
• 负责人: Merrill, Rodney
• 金额: $ 2.59万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2006
• 资助国家: 加拿大
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=326055
• 关键词: Automated; microbiology; growth; curve; analysis

The common need for many Life Scientists is the ability to measure the growth rates of microbial cells by monitoring the increase in turbidity with time using a table-top absorption spectrometer.  However, this approach is limited in that the sample volume is relatively large (1 mL) and only a single sample can be measured at a time.  Furthermore, a sample must be taken from a growing culture to read in the spectrometer.  This application is for an automated spectrometer that directly measures microbial growth for as many as 200 separate cultures in a multi-well plate where each sample is only 0.2 mL.  This instrument functions as a thermally controlled shaker/incubator that will automatically read the turbidity values (absorbances) of the growing cell cultures at programmed time intervals and it transfers the entire datasets for 200 growth curves to a computer for analysis and plotting.  This instrument will enable researchers at the University of Guelph to amplify their research productivity by nearly 200-fold.

对于许多生命科学家来说，共同的需求是能够通过使用桌面吸收光谱仪监测微生物细胞的浊度随时间的增加来测量微生物细胞的生长速度。然而，这种方法的局限性在于样品体积相对较大(1毫升)，而且一次只能测量一个样品。此外，必须从正在生长的培养物中提取样本才能在光谱仪上读取。*这项应用是为了一台自动光谱仪，它可以直接测量多孔板中多达200种不同培养物的微生物生长情况，每个样品只有0.2毫升。*这台仪器的功能是一个热控摇床/培养箱，它将以编程的时间间隔自动读取正在生长的细胞培养物的浊度值(吸光度)，并将200条生长曲线的整个数据集传输到计算机进行分析和绘图。该仪器将使圭尔夫大学的研究人员能够将他们的研究效率放大近200倍。