Temperature-controllable and oxygen-free french press system

一种温度可控、无氧的法式压滤系统能够在厌氧条件下快速无创地破坏微生物细胞。与超声波等其他方法（~50%）相比，它提供了几乎完全的小细胞破坏（一次通过bbb90 %）。目前，滑铁卢大学生物系还没有这样的细胞破坏系统。我们建议购买一个温度可控和无氧的法式压滤系统，以帮助从超嗜热微生物和酵母细胞中纯化和表征氧敏感酶。该系统也可用于其他类型的细胞，如细菌和其他真核细胞，根据需要和细胞可用性，它可以处理1至35毫升的样品体积。提出的温度可控无氧法压机系统将加快氧敏感酶样品制备过程，从而更有效地利用资源，缩短培训时间，并将加速研究进展，从而提高我们在滑铁卢大学的培训项目的质量。

A temperature-controllable and oxygen-free French Press system is capable of rapidly disrupting microbial cells non-invasively under anaerobic conditions.  It provides a nearly complete breakage of small cells (>90% with one pass) compared to other methods such as sonication (~50%). Currently, there is no such system for cell disruption in the Department of Biology at the University of Waterloo.  We propose to purchase a temperature-controllable and oxygen-free French Press system to aid in the purification and characterization of oxygen-sensitive enzymes from hyperthermophilic microorganisms and yeast cells.  This system can also be used for other types of cells, such as bacterial and other eukaryotic cells, and it can handle sample-volumes from 1 to 35 ml depending on the need and cell availability. The proposed temperature-controllable and oxygen-free French Press system will speed up oxygen-sensitive enzyme sample preparation procedures, resulting in more efficient use of resources and reduced training time, and will accelerate research progress, thus increasing quality of our training programs at the University of Waterloo.