Ultracentrifuge and rotors

一个超离心机和三个不同的转子被要求支持5个不同的nserc资助的研究项目，目的是：(A)了解调节植物低温耐受性的细胞、分子和遗传机制，(B)利用细胞和动物模型确定调节胎盘交换的细胞信号机制，(C)确定参与病毒基因表达调控和新疫苗策略开发的分子因素，(D)表征肝细胞受体在从脂蛋白中选择性摄取酯化胆固醇中的作用。(E)了解植物生长素信号传导的分子和遗传机制。该设备还将支持其他几个需要偶尔使用该设备的nserc资助的项目。我们研究的一个重要部分依赖于细胞器的分离，如细胞核、叶绿体、质膜、微粒体和细胞质溶胶进行生化和分子分析。脂蛋白代谢的研究需要基于密度的脂蛋白类的连续分离，这需要长时间的高速离心。病毒颗粒分离需要大量的原料和大量的体积。有超过25名研究人员和学生参与了这5个NSERC项目，超离心机被认为是他们工作中必不可少的设备。这台设备的可用性现在已经成为他们研究的限制因素，因为两个超离心机和几个转子已经从我们的设施中取消了。如果得到资助，新的超离心机将每天使用。它将位于中心位置，并向我们部门的其他用户开放，就像我们的公共资助设备一样。迫切需要的是15,000美元的部门捐款（附信）和需要超离心的不同研究团队的15,000美元。

An ultracentrifuge and three different rotors are requested to support 5 different NSERC-funded research programs which aim : (A) to understand the cellular, molecular, and genetic mechanisms that regulate low temperature tolerance in plants, (B) to identify the cellular signalling mechanisms regulating placental exchanges using cellular and animal models, (C) to determine the molecular factors involved in the regulation of viral gene expression and the development of new vaccinal strategies, (D) to characterize the role of hepatocyte receptors in the selective uptake of esterified cholesterol from lipoproteins, (E) to understand the molecular and genetic mechanisms that regulate auxin signalling in plants. This equipment will also support the program of several other NSERC-funded programs that need to use this equipment more sporadically. An important part of our research depends on the isolation of cell organelles such as the nucleus, chloroplasts, plasma membranes, microsomes, and cytosol for biochemical and molecular analyses. Studies on lipoprotein metabolism require the serial fractionation of lipoprotein classes based on density that requires long high speed centrifugation. Virus particle isolation requires large amounts of raw material and large volumes. There are more than 25 researchers and students involved in these five NSERC programs and the ultracentrifuge is considered an essential piece of equipment for their work. The availability of this equipment has now become a limiting factor for their research since two ultracentrifuges and several rotors have been eliminated from our facilities. If funded, the new ultracentrifuge will be used on a daily basis. It will be centrally located and available to other users in our department as always the case with our publicly funded equipments. The urgent need is demonstrated by the $15,000.00 departmental contribution (letter attached) and the $15,000.00 from the different research teams that need ultracentrifugation.