Intravital microscopy system for observations on the peripheral vasculature in situ

在研究外周血管功能方面，我有一个不断发展的NSERC项目，特别是在骨骼肌功能和活动性充血方面。我还与皇后大学的Anne Croy博士、Alistair Summerlee博士和Pat Wright博士进行了卓有成效的合作，这些合作得到了NSERC和CIHR的联合资助(CHRP)，他们都在使用显微镜回答NSERC资助的研究问题，并培训高素质的人员。他说，目前的设备要求是活体内显微镜系统，包括为活体显微显微镜改装的直立式奥林巴斯显微镜，用于图像采集的相机，以及显微手术所需的立体显微镜。--再加上用我最后一笔启动资金购买的设备，将允许建造第二个显微镜站。我的实验室中已经存在这种显微镜配置，这个请求是为了一个非常需要的、具有类似配置的第二个显微镜系统。我说，请求这台设备的原因是维护和发展我目前的计划，促进目前的合作，并能够适应新的合作。实验平均5-6个小时，目前的显微镜装置每天用于3个实验，因此每天大约15-18个小时。但如此频繁的使用来自于我的实验室目前正在培训的3名研究生，以及与非常有才华的研究生和来自非常富有成效的合作的学生的前景。我目前项目的发展、合作的可持续性和未来合作的可能性取决于构建第二个显微镜系统的能力。

I have a growing NSERC program in the investigation into peripheral vascular function especially as it applies to skeletal muscle function and active hyperemia.  I also have very productive collaborations with Dr. Anne Croy at Queens' University, fostered by joint NSERC and CIHR funding (CHRP), Dr. Alistair Summerlee, and Dr. Pat Wright, all of which are using microscopy to answer NSERC funded research questions and train highly qualified personel.     The current equipment request is for an intravital microscopy system including an upright Olympus microscope modified for intravital microsopy, a camera for image acquisition, and a stereomicroscope necessary for microsurgery.  This equipment, coupled with equipment to be bought with the last of my start up funds will allow for the construction of a second microscopy station.  This microscope configuration already exists in my laboratory and this request is for a much needed, second microscopy system with a similar configuration.     The reasons for requesting this equipment is to maintain and grow my current program, to foster current collaborations and be able to accommodate new collaborations. Experiments average 5-6 hours and the current microscopy unit is being used for 3 experiments per day, therefore from approximately 15-18 hours per day.  Such heavy use comes from the 3 graduate students currently being trained in my lab, with prospects of increasing this number with very talented graduate students and students from very productive collaborations. The growth of my current program, the sustainability of collaborations and the possibility for future collaborations depend on the ability to construct a second microscopy system.