Mechanisms regulating porcine fetal survival and outcomes from therapeutic intervention

调节猪胎儿存活的机制和治疗干预的结果

• 批准号: 339701-2006
• 负责人: Croy, Anne
• 金额: $ 6.92万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Agriculture and Agri-Food Canada Research Partnership
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=363963
• 关键词: Mechanisms; regulating; porcine; fetal; survival

Prenatal mortality is an economic concern for commercial pork producers in North America. Twenty to 30% of fetuses are lost between gestation days (gd)12 and 30 and another 10 to 15% later in gestation. Placental efficiency and uterine capacity are the parameters identified as having the greatest influences on porcine fetal survival but selection strategies targeting these traits have not significantly improved litter sizes. Greater definition of the physiological and immunological interactions between the mother's uterine lining and embryos as they attach (gd!2) is needed to enable new ways of promoting developmental success of more embryos. The proposed research addresses improved molecular understanding of the peri-attachment porcine maternal-fetal interface and evaluation of a novel therapeutic approach. We established a highly successful technique for quantitative comparative studies between arresting and healthy littermates of 5 distinct tissue types within individual attachment sites from gd!9. Aim 1 of the proposal seeks the time at which heterogeneity in gene expression significant enough to alter viability is established between littermates (ie before gd!9) and if this can be modified by adjuvant treatment at first estrus. Aim 2 will characterize uterine maternal dendritic cells and their interactions with endometrial lymphocytes. Aim 3 will address cellular and cytokine outcomes within arresting attachment sites due to reduction in the CC chemokine decoy molecule D6. Ontario Pork and Bioniche Life Sciences, Inc. have committed funding support. Outcomes from this proposal should provide new insights into control of mechanisms that regulate success or failure of pregnancy in pigs and provide opportunity for economic gain in the food production and manufacturing sectors of the Canadian economy. Information from these studies should also be of value for other livestock species and for humans with pregnancy complications, infertility or using assisted reproductive technologies such as in vitro fertilization an embryo transfer.

产前死亡率是北美商业猪肉生产商的一个经济问题。20%到30%的胎儿在妊娠第12天到第30天之间丢失，另外10%到15%在妊娠后期丢失。胎盘效率和子宫容量是被确定为对猪胎儿存活影响最大的参数，但针对这些性状的选择策略并没有显着改善窝仔数。更好地定义了母亲子宫内膜和胚胎之间的生理和免疫相互作用（gd！2)需要新的方法来促进更多胚胎的发育成功。该研究旨在提高对附件周围猪母胎界面的分子理解，并评估一种新的治疗方法。我们建立了一个非常成功的技术，定量比较研究之间的逮捕和健康的同窝出生的5个不同组织类型的个人附着网站从gd！9.目的1的建议寻求在基因表达的异质性显着足以改变生存能力的时间是建立在同窝出生（即前gd！9)以及是否可以通过在第一次发情时的辅助治疗来改变。目的2研究子宫内膜树突状细胞及其与子宫内膜淋巴细胞的相互作用。目的3将解决由于CC趋化因子诱饵分子D 6的减少而引起的在阻滞附着位点内的细胞和细胞因子结果。安大略猪肉和Bioniche生命科学公司承诺提供资金支持。该提案的结果应提供新的见解，以控制机制，规范怀孕的成功或失败的猪，并提供机会，在加拿大经济的食品生产和制造业的经济收益。来自这些研究的信息对于其他牲畜物种和患有妊娠并发症、不孕症或使用体外受精和胚胎移植等辅助生殖技术的人类也应该是有价值的。