Towards structure determination in membrane proteins: fast MAS probe for sensitivity enhancement through direct proton detection

膜蛋白结构测定：快速 MAS 探针通过直接质子检测增强灵敏度

• 批准号: 358881-2008
• 负责人: Ladizhansky, Vladimir
• 金额: $ 9.32万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=364301
• 关键词: Towards; structure; determination; membrane; proteins

Solid-state nuclear magnetic resonance (NMR) is rapidly advancing as a powerful tool for structure determination in peptides and proteins, a key to the understanding of their functions. Of particular interest are membrane peptides and proteins that are not readily amenable to structural analysis by other high-resolution techniques such as X-ray crystallography and solution state NMR. Thus, solid-state NMR is the only technique that can provide definitive information on the structural detail in these systems.  The research outlined in this proposal focuses on the development of new solid-state NMR methods for structural studies aiming at the increase of sensitivity of measurements, and on the applications of these methods in biological systems of interest, such as membrane- and cytoskeleton-associated myelin basic protein, and proteorhodopsin.

固态核磁共振（NMR）作为肽和蛋白质结构测定的有力工具正在迅速发展，这是了解其功能的关键。特别感兴趣的是膜肽和蛋白质，它们不容易通过其他高分辨率技术如X射线晶体学和溶液状态NMR进行结构分析。因此，固态NMR是唯一可以提供这些系统结构细节的确定性信息的技术。本提案中概述的研究重点是开发用于结构研究的新的固态NMR方法，旨在提高测量的灵敏度，以及这些方法在感兴趣的生物系统中的应用，例如膜和细胞色素相关的髓鞘碱性蛋白，和变形视紫红质。