Multi-spectral fluorescence molecular tomography

新的生物医学成像技术的出现使我们在如何成像人类和动物方面取得了重大进展。特别是，新模式的主流出现，如漫射光学成像，使研究人员能够以无创的方式对体内血流动力学进行成像。与此同时，随着人类内源性漫射光学成像的出现，一种新的方法正在出现，能够通过荧光跟踪注射在动物体内的分子产物。这种被称为分子成像的新成像技术有着巨大的前景：尽管人们会通过解剖变化和异常来检测疾病，但我们现在可以使用新方法在活生物体的细胞和分子水平上提供生物过程的微创表征和测量。到目前为止，大多数光学成像仪都受到扩散和非均质性的限制。红外光在组织中的注射和检测（有或没有荧光）受到介质光学特性的复杂变化的影响。对于许多应用，特别是肿瘤学，有时忽略这一点就足够了，但随着研究的进展，研究人员越来越敏锐地意识到这种方法的局限性，特别是在空间分辨率和量化方面。本建议的目的是开发解决这些限制的技术。我们建议以两种方式解决这些限制：首先，通过开发多光谱荧光成像仪提供更多关于组织光学特性的信息。其次，从另一种方式（MRI）创建模板小鼠模型的字典，可以像先前一样用于重建。利用该模板上格林函数的预计算进行图像重建。从这个建议中产生的发展将通过增加其产品线量化能力使合作伙伴公司受益。

The advent of new biomedical imaging technique has enabled significant progresses in how we image humans and animals. In particular, the emergence in the mainstream of new modalities, such as diffuse optical imaging, has enabled researchers to image hemodynamics in vivo in a non invasive fashion. In parallel, associated with the emergence of endogenous diffuse optical imaging in humans, a new approach is emerging enabling to follow molecular products injected in animals, in vivo, through fluorescence. This new imaging technique, referred to as molecular imaging holds great promises: whereas one would detect diseases through anatomical changes and anomalies, we now can use new methods to provide a minimally invasive characterization and measurement of biological processes at the cellular and molecular levels of living organisms.Up until now, most optical imagers have been constrained by diffusion and heterogeneity. The injection and detection of infrared light in tissues (with and without fluorescence) is subject to the complex variations of optical properties of the medium. For many applications, in particular oncology, it is sometimes sufficient to ignore this, but as research advances, researchers are becoming more acutely aware of the limitations of this method, in particular for spatial resolution and quantification.The aim of this proposal is to develop techniques to address these limitations. We propose to address these limitations in two ways: first by developping a multi-spectral fluorescent imager providing more information about tissue optical properties. Second to create a dictionnary of template mice models  from another modality (MRI) that can be used as prior in the reconstruction. The pre- computation of Green's functions on this template will be used to perform image reconstruction.The developments originating from this proposal will benefit the partner company by increasing its product line quantification ability.