Structural investigations of protein self-assembly

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The objective of my research is to determine how proteins combine to form complex molecular machines and how proteins transmit chemical signals throughout the cell. My proposed research program is divided into three major projects: The first project studies the self-assembly the protein ClpX and its role in the degradation, or removal, of damaged proteins in the cell. The second project studies the ability of the protein SOX9 to bind DNA in different ways. SOX9 is an essential protein in male sex determination. The third project focuses upon the atomic level, structural determination of proteins involved in bacteriophage viral assembly. All of the proposed projects draw upon a combination of molecular biological and biochemical techniques including nuclear magnetic resonance spectroscopy.