Molecular architecture in gene expression and virus-host interactions

基因表达和病毒-宿主相互作用的分子结构

• 批准号: 238934-2011
• 负责人: Donaldson, Logan
• 金额: $ 3.09万
• 依托单位: York University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=524507
• 关键词: Molecular; architecture; gene; expression; virus

The aim of my research is to understand how proteins assemble into an architecture that is well suited to a specific function. The atomic level of detail required to visualize differences in protein assemblies is achieved by using techniques such as NMR spectroscopy and X-ray crystallography that are available locally to my laboratory. The three aims in my proposal cover three general areas of cell signaling. The first aim examines how a pair of Sox9 proteins working together is capable of activating a different developmental program in a cell than one Sox9 protein working alone. The second aim examines the nature of the interactions between a bacterial transcription factor and its promoters that drive antibiotic resistance. The third aim examines how a bacterial genetic program is altered during viral infection by a protein called ea8.5. The basic research described in this proposal will be used to understand the new aspects of cell signaling and help design selective therapies against disease.

我研究的目的是了解蛋白质如何组装成非常适合特定功能的结构。通过使用我实验室本地可用的 NMR 波谱学和 X 射线晶体学等技术，可以实现可视化蛋白质组装差异所需的原子级细节。我的提案中的三个目标涵盖了细胞信号传导的三个一般领域。第一个目标是研究一对 Sox9 蛋白一起工作如何能够激活细胞中与一个 Sox9 蛋白单独工作不同的发育程序。第二个目标是检查细菌转录因子与其驱动抗生素耐药性的启动子之间相互作用的性质。第三个目标是研究细菌遗传程序在病毒感染期间如何被一种名为 ea8.5 的蛋白质改变。该提案中描述的基础研究将用于了解细胞信号传导的新方面，并帮助设计针对疾病的选择性疗法。