Protein Purification Platform Upgrade for Structural Biology Research

用于结构生物学研究的蛋白质纯化平台升级

• 批准号: 422543-2012
• 负责人: Donaldson, Logan
• 金额: $ 0.95万
• 依托单位: York University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=495815
• 关键词: Protein; Purification; Platform; Upgrade; Structural

My laboratory's primary focus is the structural determination of protein complexes by NMR spectroscopy and X-ray crystallography. This research is funded by a 2011-2016 NSERC Discovery Grant and a 2011-2014 NSERC Discovery Accelerator Award. One of the most integral components of the workflow in this research environment is efficient and reproducible protein purification using a combination of manual and automated chromatography instruments. The chromatography equipment in the laboratory was purchased in 2001 with startup funds and a CFI New Opportunities award. Six months ago, the fraction collector robot on the only automated (AKTA FPLC) chromatography system failed. This proposal represents a request for funds to replace the fraction collector as well as a few minor maintenance items such as an ultraviolet lamp for the detector.

我实验室的主要重点是通过核磁共振波谱和 X 射线晶体学测定蛋白质复合物的结构。这项研究由 2011-2016 年 NSERC 发现补助金和 2011-2014 年 NSERC 发现加速器奖资助。该研究环境中工作流程中最不可或缺的组成部分之一是使用手动和自动色谱仪器的组合进行高效且可重复的蛋白质纯化。实验室的色谱设备是 2001 年用启动资金和 CFI 新机会奖购买的。六个月前，唯一的自动化 (AKTA FPLC) 色谱系统上的馏分收集机器人发生故障。该提案代表了对更换馏分收集器以及一些小型维护项目（例如检测器的紫外线灯）的资金请求。