Biomarkers for haemic neoplasia in the blue mussel, mytilus trossulus

蓝贻贝（mytilus trossulus）血液肿瘤的生物标志物

• 批准号: 349918-2006
• 负责人: Baldwin, Susan
• 金额: $ 3.3万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=365288
• 关键词: Biomarkers; haemic; neoplasia; blue; mussel

The overall objective of this research is to develop biomarkers for a leukemia-like disease in mussels called haemic neoplasia (HN). This disease has a high prevalence in Mytilus trossulus, the species native to the British Columbia coast. Although the disease likely has a virus aetiology there are some indications that susceptibility of individuals to the disease is exacerbated in the presence of pollutants. The mussel is used widely as a bioindicator organism, and is being evaluated as a potential monitoring tool for the Greater Vancouver Regional District (GVRD) receiving environment-monitoring program for one wastewater treatment plant. Haemic neoplasia is one of the various endpoints being evaluated. Our goal is to develop high throughput techniques such as gene expression analysis for monitoring of this disease. Our hypothesis is that apoptosis processes are impaired in leukemic mussel haemocytes (LMH). We propose to test this hypothesis by subjecting LMH to various apoptosis inducing agents. Knowing which pathways are dysregulated in LMH will help identify potential biomarkers for this disease. We are already monitoring the pro-apoptotic genes p53 and p63/p73 in a separate study. To extend the number of biomarker genes, and as a second objective of this proposal, we aim to sequence MDM2, an anti-apoptotic gene, and develop a quantitative polymerase chain reaction protocol so that MDM2 gene expression in LMH can be monitored also. Monitoring multiple biomarkers that include both protagonists and antagonists of a particular pathway gives more information about potential dysregulation of that pathway.

这项研究的总体目标是为贻贝中的白血病样疾病开发生物标志物，称为血液肿瘤（HN）。这种疾病在原产于不列颠哥伦比亚省海岸的紫贻贝（Mytilus trossulus）中流行率很高。虽然这种疾病可能有病毒病因，但有一些迹象表明，在污染物存在的情况下，个体对这种疾病的易感性会加剧。贻贝被广泛用作生物指示生物，并正在评估作为一个潜在的监测工具，为大温哥华地区（GVRD）接收环境监测计划的一个污水处理厂。贫血性瘤形成是正在评价的各种终点之一。我们的目标是开发高通量技术，如基因表达分析，用于监测这种疾病。我们的假设是，白血病贻贝血细胞（LMH）的凋亡过程受损。我们建议测试这一假设LMH受到各种凋亡诱导剂。了解LMH中哪些途径失调将有助于确定这种疾病的潜在生物标志物。我们已经在一项单独的研究中监测了促凋亡基因p53和p63/p73。为了扩大生物标志物基因的数量，并作为本提案的第二个目标，我们的目标是测序MDM2，一种抗凋亡基因，并开发一种定量聚合酶链反应方案，使MDM2基因表达在LMH也可以监测。监测包括特定途径的主角和拮抗剂的多种生物标志物提供了有关该途径潜在失调的更多信息。