Selenium biosensor development

硒生物传感器的开发

• 批准号: 531440-2018
• 负责人: Baldwin, Susan
• 金额: $ 1.82万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=644535
• 关键词: Selenium; biosensor; development

Selenium is an essential element for all life, but when concentrations exceed nutritional requirements it**becomes toxic. Some industrial activities, such as mining, can accelerate the release of dissolved selenium**compounds into the receiving environment. To remove dissolved selenium from industrial wastewater, reactors**are used to biologically reduce the soluble selenium species into non-soluble forms. In order to manage**selenium release at industrial sites, the concentrations of soluble selenium species in the wastewater and in the**treated water must be measured in an accurate and timely way. Currently this cannot be done on site, as the**method for measuring selenium speciation is highly specialized and can only be carried out by a few**commercial laboratories. In this project, a biotechnology company, FREDsense wants to use their patented**portable, cost-effective device for measuring contaminants of interest in water directly in the field. Their first**product was an arsenic sensor, and they want to employ a similar approach to develop a selenium biosensor.**Their technology is based on a response to genetic elements that are selective and sensitive to the analyte of**interest. Dr. Baldwin has been working with selenate-reducing organisms and her group has collected a vast**amount of metagenomic data on these. The goal of this project is to mine these metagenomic data for potential**genetic elements that can be tested for their selenium selectivity and sensitivity in FREDsense's technology.**Additionally, the selenate reducing bacteria enrichments in Dr. Baldwin's laboratory will be used for functional**screening for selenate and selenite selective genetic elements. The outcome of this work will be identification**of candidate genetic elements with the selectivity and sensitivity for dissolved selenium species, selenate and**selenite.

硒是所有生命的必需元素，但当浓度超过营养需求时，它就会变得有毒。一些工业活动，如采矿，可以加速溶解的硒 ** 化合物释放到接收环境中。为了从工业废水中去除溶解的硒，使用反应器 ** 将可溶性硒物质生物还原成不溶性形式。为了控制工业场所的硒释放，必须以准确和及时的方式测量废水和经处理的水中可溶性硒物种的浓度。目前，这还不能在现场进行，因为测量硒形态的方法是高度专业化的，只能由几个商业实验室进行。在这个项目中，一家生物技术公司，FREDsense希望使用他们的专利 ** 便携式，成本效益高的设备，用于直接在现场测量水中感兴趣的污染物。他们的第一个 ** 产品是砷传感器，他们希望采用类似的方法开发硒生物传感器。他们的技术是基于对遗传元件的反应，这些遗传元件对感兴趣的分析物具有选择性和敏感性。鲍德温博士一直在研究能减少硒酸盐的生物体，她的研究小组已经收集了大量关于这些生物体的宏基因组数据。该项目的目标是挖掘这些宏基因组数据，以寻找潜在的遗传元件，这些遗传元件可以在FREDsense技术中测试其硒选择性和敏感性。此外，鲍德温博士实验室中的硒酸盐还原细菌富集物将用于功能性筛选硒酸盐和亚硒酸盐选择性遗传元件。这项工作的结果将是鉴定 ** 候选遗传元件的选择性和敏感性溶解硒物种，硒酸盐和 ** 亚硒酸盐。