Real time RT-PCR for advanced tissue engineering

组织工程和再生医学的前景已经激发了我们的想象力。组织工程跨越了传统上截然不同的研究领域，包括聚合物科学与工程、细胞生物学和外科之间的基础研究。许多多学科的合作、出版物和拨款证明，Shoichet、Morhead和Radisic的研究实验室都在这个界面上。重要的是，Shoichet和Morhead在中枢神经系统创伤后(如中风或脊髓损伤后)的再生和药物输送策略上积极合作。同样，Radisic和Shoichet积极合作设计用于细胞指导的支架，特别是用于心脏应用的支架。我们成功组织工程策略的关键是设计考虑生物学的支架和输送工具-具体提供将促进细胞和组织再生的适当化学和物理刺激。我们研究的目标是促进组织工程的基础科学和使能技术。我们的目标是专门设计适合组织再生的环境。我们在基础材料科学和应用于生物学的工程方面的研究计划可能会为促进脑、脊髓或心脏损伤后的修复提供关键。为了实现这些目标，我们组成了由工程师、科学家和外科医生组成的多学科团队。为了实现这些多学科研究目标，需要多学科设施。我们需要实时、逆转录聚合酶链式反应、RT-PCR仪器，以便我们能够在组织工程的背景下回答关于细胞命运、分化和生存的关键问题。我们的最终目标是将我们的关键发现转化为临床应用和商业应用，造福加拿大人。

The promise of tissue engineering and regenerative medicine has captured our imagination.  Tissue Engineering cuts across traditionally distinct research areas encompassing fundamental research at the interface of polymer science & engineering, cell biology and surgery.  The research laboratories of Shoichet, Morshead and Radisic are at this interface as evidenced by numerous multi-disciplinary collaborations, publications and grants. Importantly, Shoichet and Morshead actively collaborate on regenerative and drug delivery strategies after traumatic injury in the central nervous system, such as after stroke or spinal cord injury.  Similarly, Radisic and Shoichet actively collaborate on a scaffolds designed for cell guidance, particularly for cardiac applications.  The key to our successful tissue engineering strategies is the design of scaffolds and delivery vehicles with biology in mind - that is specifically providing the appropriate chemical and physical stimuli that will promote cell and tissue regeneration.     The objectives of our research are to advance the basic science and enabling technologies of Tissue Engineering.  Our goal is to specifically design an environment suitable for tissue regeneration. Our research programs in fundamental materials science and engineering applied to biology may provide the key to promoting repair after injury in the brain, spinal cord or heart.  To achieve these goals, we form multi-disciplinary teams of engineers, scientists and surgeons.     To accomplish these multi-disciplinary research goals, multi-disciplinary facilities are required.  We require a real-time, reverse-transcriptase polymerase chain reaction, RT-PCR instrument so that we can answer critical questions about cell fate, differentiation and survival in the context of tissue engineering. Our ultimate goal is to translate our key findings to clinical use and commercial application for the benefit of Canadians.