Real time RT-PCR for advanced tissue engineering

组织工程和再生医学的前景吸引了我们的想象力。组织工程跨越了传统上不同的研究领域，包括聚合物科学与工程、细胞生物学和外科学的基础研究。Shoichet， Morshead和radicic的研究实验室在这个界面上，许多多学科合作，出版物和资助证明了这一点。重要的是，Shoichet和Morshead在中枢神经系统创伤性损伤（如中风或脊髓损伤）后的再生和药物递送策略方面积极合作。同样，radicic和Shoichet积极合作开发一种用于细胞引导的支架，特别是用于心脏应用。我们成功的组织工程策略的关键是在设计支架和运载工具时考虑到生物学，即提供适当的化学和物理刺激，以促进细胞和组织再生。我们的研究目标是推进组织工程的基础科学和使能技术。我们的目标是专门设计适合组织再生的环境。我们在基础材料科学和应用于生物学的工程方面的研究项目可能为促进脑、脊髓或心脏损伤后的修复提供关键。为了实现这些目标，我们组建了由工程师、科学家和外科医生组成的多学科团队。为了实现这些多学科的研究目标，需要多学科的设施。我们需要一个实时的，逆转录聚合酶链反应，RT-PCR仪器，这样我们就可以回答组织工程背景下关于细胞命运，分化和生存的关键问题。我们的最终目标是将我们的主要发现转化为临床应用和商业应用，以造福加拿大人。

The promise of tissue engineering and regenerative medicine has captured our imagination.  Tissue Engineering cuts across traditionally distinct research areas encompassing fundamental research at the interface of polymer science & engineering, cell biology and surgery.  The research laboratories of Shoichet, Morshead and Radisic are at this interface as evidenced by numerous multi-disciplinary collaborations, publications and grants. Importantly, Shoichet and Morshead actively collaborate on regenerative and drug delivery strategies after traumatic injury in the central nervous system, such as after stroke or spinal cord injury.  Similarly, Radisic and Shoichet actively collaborate on a scaffolds designed for cell guidance, particularly for cardiac applications.  The key to our successful tissue engineering strategies is the design of scaffolds and delivery vehicles with biology in mind - that is specifically providing the appropriate chemical and physical stimuli that will promote cell and tissue regeneration.     The objectives of our research are to advance the basic science and enabling technologies of Tissue Engineering.  Our goal is to specifically design an environment suitable for tissue regeneration. Our research programs in fundamental materials science and engineering applied to biology may provide the key to promoting repair after injury in the brain, spinal cord or heart.  To achieve these goals, we form multi-disciplinary teams of engineers, scientists and surgeons.     To accomplish these multi-disciplinary research goals, multi-disciplinary facilities are required.  We require a real-time, reverse-transcriptase polymerase chain reaction, RT-PCR instrument so that we can answer critical questions about cell fate, differentiation and survival in the context of tissue engineering. Our ultimate goal is to translate our key findings to clinical use and commercial application for the benefit of Canadians.