Real time RT-PCR for advanced tissue engineering

用于先进组织工程的实时 RT-PCR

• 批准号: 359539-2008
• 负责人: Shoichet, Molly
• 金额: $ 8.19万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=365391
• 关键词: Real; time; RT; PCR; advanced

The promise of tissue engineering and regenerative medicine has captured our imagination.  Tissue Engineering cuts across traditionally distinct research areas encompassing fundamental research at the interface of polymer science & engineering, cell biology and surgery.  The research laboratories of Shoichet, Morshead and Radisic are at this interface as evidenced by numerous multi-disciplinary collaborations, publications and grants. Importantly, Shoichet and Morshead actively collaborate on regenerative and drug delivery strategies after traumatic injury in the central nervous system, such as after stroke or spinal cord injury.  Similarly, Radisic and Shoichet actively collaborate on a scaffolds designed for cell guidance, particularly for cardiac applications.  The key to our successful tissue engineering strategies is the design of scaffolds and delivery vehicles with biology in mind - that is specifically providing the appropriate chemical and physical stimuli that will promote cell and tissue regeneration.     The objectives of our research are to advance the basic science and enabling technologies of Tissue Engineering.  Our goal is to specifically design an environment suitable for tissue regeneration. Our research programs in fundamental materials science and engineering applied to biology may provide the key to promoting repair after injury in the brain, spinal cord or heart.  To achieve these goals, we form multi-disciplinary teams of engineers, scientists and surgeons.     To accomplish these multi-disciplinary research goals, multi-disciplinary facilities are required.  We require a real-time, reverse-transcriptase polymerase chain reaction, RT-PCR instrument so that we can answer critical questions about cell fate, differentiation and survival in the context of tissue engineering. Our ultimate goal is to translate our key findings to clinical use and commercial application for the benefit of Canadians.

组织工程和再生医学的前景已经吸引了我们的想象力。组织工程跨越了传统上不同的研究领域，包括聚合物科学与工程，细胞生物学和外科学的基础研究。Shoichet，Morshead和Radisic的研究实验室在这个界面上，众多的多学科合作，出版物和赠款证明了这一点。重要的是，Shoichet和Morshead积极合作研究中枢神经系统创伤性损伤后的再生和药物递送策略，例如中风或脊髓损伤后。同样，Radisic和Shoichet积极合作研究用于细胞引导的支架，我们成功的组织工程策略的关键是设计具有生物学意义的支架和运载工具-即专门提供适当的化学和物理刺激，促进细胞和组织再生。 我们的研究目标是推进组织工程的基础科学和使能技术。我们的目标是专门设计一个适合组织再生的环境。我们的基础材料科学和工程应用于生物学的研究项目可能为促进大脑，脊髓或心脏损伤后的修复提供关键。为了实现这些目标，我们组建了工程师，科学家和外科医生的多学科团队。 为了实现这些多学科的研究目标，需要多学科的设备。我们需要一个实时的，逆转录酶聚合酶链反应，RT-PCR仪器，这样我们就可以回答组织工程背景下细胞命运，分化和存活的关键问题。我们的最终目标是将我们的关键发现转化为临床应用和商业应用，以造福加拿大人。