Real time RT qPCR equipment ( QS5 QPCR System , Thermo Fisher Scientific)

作为一个合作研究团队，我们正在申请Q5S qPCR系统（定量聚合酶链反应）来加强我们联邦资助的研究项目，旨在改善药物开发、配方、给药和治疗效果。我们实验室目前的设备已经使用了20多年，由于持续存在的技术问题和与现代基因表达研究的试剂混合物不兼容，需要更换。至关重要的是，我们将现有的qPCR机器替换为新的，因为该仪器对我们合作中未完成的实验至关重要。与不同供应商销售的其他型号相比，Q5S qPCR系统具有通用性，可以满足我们项目中所有研究人员的要求。我们的合作研究项目，包括博士。Mishra、Hoare、Selvaganapathy、Gupta和Niles是由NSERC、CIHR、CHRP、补助金和NSERC的发现补助金计划资助的。Drs。Hoare和Selvaganapathy是其他RTI拨款的共同申请人，与这里所要求的设备无关；因此，它们不能在此应用程序中列出。我们由化学工程、机械工程、生物、精神病学和行为神经科学系的教员组成。我们的研究项目包括药物开发、配方和给药，包括针对中枢神经系统疾病的鼻内给药纳米技术平台。实时qPCR仪器将用于在疾病状态下被改变的基因表达的定量，并由我们开发的新药和药物制剂调节。测量基因表达的实验将在各种脑部疾病的细胞、蠕虫和临床前啮齿动物模型中进行。麦克马斯特大学附近的实验室没有类似的设备；因此，该仪器的采购对于进行我们的实验和推进我们联邦资助的合作研究项目至关重要。该仪器的可用性将显著推进我们的创新研究计划，促进实验合作，以开发新的药物化合物和改善药物给药到大脑。这将最终导致提高治疗效果，旨在达到治疗神经退行性疾病的临床阶段。此外，我们的研究将显著有助于该领域对新型神经营养因子及其分子机制的理解。通过增加使用当前行业标准机器的可访问性和经验，获得该仪器将立即使我们所有的学生受益。最终，从我们的研究项目中获得的结果将大大有利于加拿大和国际社会的知识转化和传播新的治疗方法的可用性，以治疗脑部疾病

As a collaborative research team, we are requesting the Q5S qPCR system (quantitative- polymerase chain reaction) to enhance our federally funded research programs aimed at improving drug development, formulations, delivery and therapeutic efficacy. The current equipment in our laboratory is more than 20 years old and needs replacing due to on-going technical issues and incompatibilities with modern reagent mixtures for gene expression studies. It is critical that we replace our current qPCR machine for a new one as this instrument is essential to the experiments underdone in our collaborations. The Q5S qPCR system is versatile as compared to other models marketed by different suppliers and meets the requirements of all the researchers involved in our projects. Our collaborative research programs, which include Drs. Mishra, Hoare, Selvaganapathy, Gupta and Niles, are funded by NSERC, CIHR, CHRP, grants and NSERC's Discovery Grants program. Drs. Hoare and Selvaganapathy are co--applicants on other RTI grants not related to the equipment requested here; therefore, they cannot be listed on this application. Together, we consist of faculty members representing the departments of Chemical Engineering, Mechanical Engineering, Biology, and Psychiatry and Behavioral Neurosciences. Our research programs encompass drug development, formulation and delivery, including a nanotechnology platform for intranasal drug delivery targeting central nervous system disorders. The real--time qPCR instrument will be used for the quantification of gene expression for genes that are altered in disease states and regulated by novel drugs and drug formulations we have developed. Experiments measuring gene expression will be performed in cellular, worm and pre-clinical rodent models of various brain disorders. Similar equipment is not available in nearby laboratories at McMaster; therefore, procurement of this instrument is essential for conducting our experiments and the advancement of our federally funded collaborative research programs. The availability of this instrument will significantly advance our innovative research programs and promote the collaboration of experiments with the aim of developing novel drug compounds and improving drug delivery to the brain. This will ultimately result in increased therapeutic efficacies aimed to reach clinical stages for the treatment of neurodegenerative disorders. Furthermore, our research will significantly contribute to the field's understanding of novel neurotrophic factors and their molecular mechanisms. Acquiring this instrument will immediately benefit all our students through an increase in accessibility and experience using current industry-standard machines. Ultimately, the results obtained from our research programs will significantly benefit Canada and the international community with regards to knowledge translation and dissemination of the availability of new therapeutics for the treatment of brain disorders.**