Real time RT qPCR equipment ( QS5 QPCR System , Thermo Fisher Scientific)

实时 RT qPCR 设备（QS5 QPCR 系统，Thermo Fisher Scientific）

• 批准号: RTI-2020-00850
• 负责人: Mishra, Ram
• 金额: $ 2.29万
• 依托单位: McMaster University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=684149
• 关键词: Real; time; RT; qPCR; equipment

As a collaborative research team, we are requesting the Q5S qPCR system (quantitative- polymerase chain reaction) to enhance our federally funded research programs aimed at improving drug development, formulations, delivery and therapeutic efficacy. The current equipment in our laboratory is more than 20 years old and needs replacing due to on-going technical issues and incompatibilities with modern reagent mixtures for gene expression studies. It is critical that we replace our current qPCR machine for a new one as this instrument is essential to the experiments underdone in our collaborations. The Q5S qPCR system is versatile as compared to other models marketed by different suppliers and meets the requirements of all the researchers involved in our projects. Our collaborative research programs, which include Drs. Mishra, Hoare, Selvaganapathy, Gupta and Niles, are funded by NSERC, CIHR, CHRP, grants and NSERC's Discovery Grants program. Drs. Hoare and Selvaganapathy are co--applicants on other RTI grants not related to the equipment requested here; therefore, they cannot be listed on this application. Together, we consist of faculty members representing the departments of Chemical Engineering, Mechanical Engineering, Biology, and Psychiatry and Behavioral Neurosciences. Our research programs encompass drug development, formulation and delivery, including a nanotechnology platform for intranasal drug delivery targeting central nervous system disorders. The real--time qPCR instrument will be used for the quantification of gene expression for genes that are altered in disease states and regulated by novel drugs and drug formulations we have developed. Experiments measuring gene expression will be performed in cellular, worm and pre-clinical rodent models of various brain disorders. Similar equipment is not available in nearby laboratories at McMaster; therefore, procurement of this instrument is essential for conducting our experiments and the advancement of our federally funded collaborative research programs. The availability of this instrument will significantly advance our innovative research programs and promote the collaboration of experiments with the aim of developing novel drug compounds and improving drug delivery to the brain. This will ultimately result in increased therapeutic efficacies aimed to reach clinical stages for the treatment of neurodegenerative disorders. Furthermore, our research will significantly contribute to the field's understanding of novel neurotrophic factors and their molecular mechanisms. Acquiring this instrument will immediately benefit all our students through an increase in accessibility and experience using current industry-standard machines. Ultimately, the results obtained from our research programs will significantly benefit Canada and the international community with regards to knowledge translation and dissemination of the availability of new therapeutics for the treatment of brain disorders.**

作为一个合作研究团队，我们要求Q5 S qPCR系统（定量聚合酶链反应），以加强我们的联邦资助的研究计划，旨在改善药物开发，配方，交付和治疗效果。我们实验室目前的设备已经使用了20多年，由于持续的技术问题以及与基因表达研究的现代试剂混合物不兼容，需要更换。我们必须更换现有的qPCR机器，因为该仪器对于我们合作中未完成的实验至关重要。与不同供应商销售的其他型号相比，Q5 S qPCR系统具有多功能性，可满足参与我们项目的所有研究人员的要求。我们的合作研究项目，其中包括博士米什拉，霍尔，塞尔瓦加纳帕蒂，古普塔和奈尔斯，由NSERC，CIHR，CHRP，赠款和NSERC的发现赠款计划资助。Hoare博士和Selvaganapathy博士是与此处要求的设备无关的其他RTI赠款的共同申请人;因此，他们不能在此申请中列出。我们由代表化学工程，机械工程，生物学，精神病学和行为神经科学部门的教职员工组成。我们的研究项目包括药物开发，配方和交付，包括针对中枢神经系统疾病的鼻内给药纳米技术平台。该真实的-时间qPCR仪器将用于定量基因表达的基因改变疾病状态和调节的新药和药物制剂，我们已经开发。测量基因表达的实验将在各种脑部疾病的细胞、蠕虫和临床前啮齿动物模型中进行。麦克马斯特附近的实验室没有类似的设备;因此，采购这种仪器对于进行我们的实验和推进我们的联邦资助的合作研究计划至关重要。该仪器的可用性将大大推进我们的创新研究计划，并促进实验合作，旨在开发新型药物化合物和改善药物向大脑的输送。这将最终导致旨在达到治疗神经退行性疾病的临床阶段的治疗功效增加。此外，我们的研究将大大有助于该领域的新的神经营养因子及其分子机制的理解。获得这种仪器将立即受益于我们所有的学生通过增加可访问性和经验，使用当前的行业标准的机器。最终，从我们的研究计划中获得的结果将使加拿大和国际社会在知识翻译和传播治疗脑部疾病的新疗法方面受益匪浅。