The evolution of shikimate associated metabolic enzymes

莽草酸相关代谢酶的进化

基本信息

  • 批准号:
    RGPIN-2020-06052
  • 负责人:
  • 金额:
    $ 3.06万
  • 依托单位:
  • 依托单位国家:
    加拿大
  • 项目类别:
    Discovery Grants Program - Individual
  • 财政年份:
    2022
  • 资助国家:
    加拿大
  • 起止时间:
    2022-01-01 至 2023-12-31
  • 项目状态:
    已结题

项目摘要

In our study, we plan to use structural biology, biochemistry and molecular biology to understand how enzymes of the shikimate pathway have evolved their respective function. Based on this approach, we will functionally annotate classes of proteins whose biological function has not been previously determined. We will target plant and microbial enzymes that stem from the shikimate pathway. There are three main objectives in this proposal: 1) Investigate the evolution of function of SKL1 in chloroplast development. The shikimate pathway plays a central role for the biosynthesis of aromatic compounds in plants and microorganisms. The pathway leads to growth hormones, aromatic amino acids, flavonoids, lignin and other central metabolites in plants. As a result, this pathway is targeted for the development of inhibitors that could function as herbicide or antimicrobial agents. SKL1 is conserved across plant species analyzed, thus the functional annotation of this protein will provide opportunities to engineer crop plants to enhance their photosynthetic efficiency and thus increase their biomass. Inactivating SKL1 directly affects plant growth, therefore an SKL1 inhibitor could function as an effective herbicide. 2) Investigate the biosynthesis and role of Chlorogenic Acids (CGAs) in protecting plants against pathogens. Recently, we functionally annotated proteins involved in CGAs biosynthesis in plants. Although, it was known that plants produce chlorogenic acids, the early steps of the metabolic pathway had not been worked out. CGAs play an important role in our diet, they are powerful antioxidant and are associated with numerous health benefits including aging and protection against cardiovascular diseases. CGAs have powerful antifungal and antifeeding properties, therefore our ability to modulate the accumulation of CGAs at different developmental stages of fruit ripening is economically important. Our studies on CGA biosynthesis in plants will provide opportunities for crop protection. 3) Investigate the role of PCA in facilitating Listeria spp. interaction within the microbiome. We have elucidated the metabolic pathway for the utilization of quinate and shikimate for the production of protocatechuate; it is hypothesized that this compound is used by Listeria species to facilitate their interaction with other organisms in the environment. Decaying green vegetables have high quinate and shikimate content which is implicated in contributing to the growth of Listeria species. We therefore plan to develop strategies to disrupt Listeria interaction with other microorganisms and thus inhibit its ability to contaminate our food sources. The results from these studies will provide a better understanding of this interaction and opportunities to prevent Listeria contamination of food supply.
在我们的研究中,我们计划利用结构生物学、生物化学和分子生物学来了解莽草酸途径的酶是如何进化其各自的功能的。基于这种方法,我们将对生物学功能尚未确定的蛋白质类进行功能性注释。我们将以源于莽草酸途径的植物和微生物酶为目标。本研究主要有三个目的:1)研究SKL1在叶绿体发育中的功能演变。莽草酸途径在植物和微生物芳香族化合物的生物合成中起着核心作用。该途径导致植物生长激素、芳香氨基酸、类黄酮、木质素和其他中心代谢物。因此,这一途径是开发可作为除草剂或抗菌剂的抑制剂的目标。SKL1在被分析的植物物种中是保守的,因此对该蛋白的功能注释将为设计作物提供机会,以提高它们的光合效率,从而增加它们的生物量。SKL1的失活直接影响植物的生长,因此SKL1抑制剂可以作为有效的除草剂。2)研究绿原酸(Chlorogenic Acids, CGAs)的生物合成及其在植物抗病中的作用。最近,我们对植物中参与CGAs生物合成的蛋白进行了功能注释。虽然人们知道植物会产生绿原酸,但代谢途径的早期步骤还没有弄清楚。CGAs在我们的饮食中扮演着重要的角色,它们是强大的抗氧化剂,与许多健康益处有关,包括衰老和预防心血管疾病。CGAs具有强大的抗真菌和抗食性,因此我们调节果实成熟不同发育阶段CGAs积累的能力具有重要的经济意义。我们对植物中CGA生物合成的研究将为作物保护提供机会。3)研究PCA在促进微生物组内李斯特菌相互作用中的作用。阐明了利用奎宁酸盐和莽草酸盐生产原儿茶酸盐的代谢途径;据推测,这种化合物被李斯特菌用来促进它们与环境中其他生物的相互作用。腐烂的绿色蔬菜含有大量的奎宁酸盐和草酸盐,这与李斯特菌的生长有关。因此,我们计划制定策略来破坏李斯特菌与其他微生物的相互作用,从而抑制其污染我们食物来源的能力。这些研究的结果将更好地了解这种相互作用,并为防止李斯特菌污染食品供应提供机会。

项目成果

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Christendat, Dinesh其他文献

Structurally diverse dehydroshikimate dehydratase variants participate in microbial quinate catabolism
  • DOI:
    10.1111/mmi.13542
  • 发表时间:
    2017-01-01
  • 期刊:
  • 影响因子:
    3.6
  • 作者:
    Peek, James;Roman, Joseph;Christendat, Dinesh
  • 通讯作者:
    Christendat, Dinesh
Structural and Mechanistic Analysis of a Novel Class of Shikimate Dehydrogenases: Evidence for a Conserved Catalytic Mechanism in the Shikimate Dehydrogenase Family
  • DOI:
    10.1021/bi200586y
  • 发表时间:
    2011-11-11
  • 期刊:
  • 影响因子:
    2.9
  • 作者:
    Peek, James;Lee, John;Christendat, Dinesh
  • 通讯作者:
    Christendat, Dinesh
Elevated tyrosine results in the cytosolic retention of 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase in Arabidopsis thaliana
  • DOI:
    10.1111/tpj.15590
  • 发表时间:
    2021-12-05
  • 期刊:
  • 影响因子:
    7.2
  • 作者:
    Kanaris, Michael;Poulin, Jimmy;Christendat, Dinesh
  • 通讯作者:
    Christendat, Dinesh
Identification of Novel Polyphenolic Inhibitors of Shikimate Dehydrogenase (AroE)
  • DOI:
    10.1177/1087057114527127
  • 发表时间:
    2014-08-01
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Peek, James;Shi, Thomas;Christendat, Dinesh
  • 通讯作者:
    Christendat, Dinesh
Structure of Arabidopsis dehydroquinate dehydratase-shikimate dehydrogenase and implications for metabolic channeling in the shikimate pathway
  • DOI:
    10.1021/bi060366
  • 发表时间:
    2006-06-27
  • 期刊:
  • 影响因子:
    2.9
  • 作者:
    Singh, Sasha Anna;Christendat, Dinesh
  • 通讯作者:
    Christendat, Dinesh

Christendat, Dinesh的其他文献

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{{ truncateString('Christendat, Dinesh', 18)}}的其他基金

High capacity protein expression and production incubators
高容量蛋白质表达和生产培养箱
  • 批准号:
    RTI-2023-00467
  • 财政年份:
    2022
  • 资助金额:
    $ 3.06万
  • 项目类别:
    Research Tools and Instruments
The evolution of shikimate associated metabolic enzymes
莽草酸相关代谢酶的进化
  • 批准号:
    RGPIN-2020-06052
  • 财政年份:
    2021
  • 资助金额:
    $ 3.06万
  • 项目类别:
    Discovery Grants Program - Individual
The evolution of shikimate associated metabolic enzymes
莽草酸相关代谢酶的进化
  • 批准号:
    RGPIN-2020-06052
  • 财政年份:
    2020
  • 资助金额:
    $ 3.06万
  • 项目类别:
    Discovery Grants Program - Individual
Functional analysis of shikimate pathway gene duplicates
莽草酸途径基因重复的功能分析
  • 批准号:
    RGPIN-2015-06747
  • 财政年份:
    2019
  • 资助金额:
    $ 3.06万
  • 项目类别:
    Discovery Grants Program - Individual
Functional analysis of shikimate pathway gene duplicates
莽草酸途径基因重复的功能分析
  • 批准号:
    RGPIN-2015-06747
  • 财政年份:
    2018
  • 资助金额:
    $ 3.06万
  • 项目类别:
    Discovery Grants Program - Individual
Functional analysis of shikimate pathway gene duplicates
莽草酸途径基因重复的功能分析
  • 批准号:
    RGPIN-2015-06747
  • 财政年份:
    2017
  • 资助金额:
    $ 3.06万
  • 项目类别:
    Discovery Grants Program - Individual
Functional analysis of shikimate pathway gene duplicates
莽草酸途径基因重复的功能分析
  • 批准号:
    RGPIN-2015-06747
  • 财政年份:
    2016
  • 资助金额:
    $ 3.06万
  • 项目类别:
    Discovery Grants Program - Individual
Functional analysis of shikimate pathway gene duplicates
莽草酸途径基因重复的功能分析
  • 批准号:
    RGPIN-2015-06747
  • 财政年份:
    2015
  • 资助金额:
    $ 3.06万
  • 项目类别:
    Discovery Grants Program - Individual
Regulation of Arabidopsis shikimate pathway
拟南芥莽草酸途径的调控
  • 批准号:
    250203-2013
  • 财政年份:
    2013
  • 资助金额:
    $ 3.06万
  • 项目类别:
    Discovery Grants Program - Individual
Regulation of the shikimate pathways in Arabidopsis
拟南芥中莽草酸途径的调控
  • 批准号:
    250203-2006
  • 财政年份:
    2012
  • 资助金额:
    $ 3.06万
  • 项目类别:
    Discovery Grants Program - Individual

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