Control of plant morphogenesis by the blade-on-petiole genes in arabidopsis

拟南芥叶柄叶片基因对植物形态发生的控制

• 批准号: 327195-2006
• 负责人: Hepworth, Shelley
• 金额: $ 2.91万
• 依托单位: Carleton University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=397626
• 关键词: Control; plant; morphogenesis; blade; petiole

A remarkable degree of architectural diversity exists between plant species. An important goal of plant developmental biologists has been to identify genes that control patterning and to understand how these genes sculpt plant organs. My laboratory studies how genes control shape and form with an emphasis on understanding the molecular mechanisms that control leaf and floral patterning in the model plant species Arabidopsis thaliana. Little is currently known about how spatial cues regulate growth asymmetry, a crucial aspect of patterning.  I have recently identified a role for two NONEXPRESSOR OF PATHOGENESIS-RELATED PROTEINS1 (NPR1)-like signaling proteins, BLADE-ON-PETIOLE (BOP) 1 and 2 in this process.  NPR1 and its role in plant defense is well-studied.  Pathogen attack leads to the accumulation of a signal molecule salicylic acid (SA) followed by altered intracellular redox potential.  NPR1 responds to changes in redox-state by becoming monomeric in form, entering the nucleus, and interacting with TGA (TGACG sequence-specific binding) transcription factors which then bind to SA-response elements in the promoters of defense genes.  The BOP proteins are structurally related to NPR1 and our preliminary analyses suggest that they function in a signaling cascade that is mechanistically similar to that of NPR1.  We intend to further examine the mechanism of BOP signaling, to identify proteins that interact with BOP1/2, to identify modifiers and downstream targets of BOP activity, and to determine how BOP interacts with other genes that regulate leaf and floral patterning.  This work will provide fresh insight into how bilateral patterning of leaves and flowers is governed and give valuable molecular details about a possible redox-control mechanism that functions in plant development.  Ultimately, our work will generate valuable tools for the manipulation of leaf and floral form and provide insights into the molecular basis of plant architectural diversity.

植物物种之间存在着显著的结构多样性。植物发育生物学家的一个重要目标是确定控制模式的基因，并了解这些基因如何塑造植物器官。我的实验室研究基因如何控制形状和形式，重点是了解控制模式植物拟南芥（Arabidopsis thaliana）叶和花图案的分子机制。目前对空间线索如何调节生长不对称性知之甚少，这是模式化的一个重要方面。我最近确定了两种非表达致病相关蛋白1（NPR 1）样信号蛋白的作用，NPR 1及其在植物防御中的作用已被充分研究。病原体攻击导致信号分子水杨酸（SA）的积累。随后改变细胞内氧化还原电位。NPR 1通过变成单体形式，进入细胞核，与TGA相互作用（TGACG序列特异性结合）转录因子，其然后结合SA-1。BOP蛋白在结构上与NPR 1相关，我们的初步分析表明它们在信号级联中起作用，我们打算进一步研究BOP信号传导的机制，以鉴定与BOP 1/2相互作用的蛋白质，以鉴定BOP活性的修饰剂和下游靶点，并确定BOP如何与其他调控叶和花图案的基因相互作用。这项工作将为叶和花的双边图案是如何管理的提供新的见解，并提供有价值的最终，我们的工作将产生有价值的工具，用于操纵叶和花的形式，并提供深入了解植物结构多样性的分子基础。