Single cell genetic profiling to reveal molecular and cellular changes in BRCA preneoplastic tissue

单细胞基因分析揭示 BRCA 癌前组织的分子和细胞变化

• 批准号: nhmrc : 1100807
• 负责人: Dr Bhupinder Pal
• 金额: $ 13.53万
• 依托单位: The Walter and Eliza Hall Institute of Medical Research
• 依托单位国家: 澳大利亚
• 项目类别: Project Grants
• 财政年份: 2016
• 资助国家: 澳大利亚
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/single-cell-genetic-preneoplastic-tissue/662528
• 关键词: Single; cell; genetic; profiling; reveal

The initial molecular and cellular events that lead to breast cancer in women with BRCA1 or BRCA2 mutations are unknown. We will use state-of-the-art genomic tools (Single Cell RNA-seq and whole genome sequencing) to determine how cancer begins in absence of normal BRCA genes. Single cell genomic profiling of stem and daughter cells from pre-cancerous breast tissue will be used to identify early-indicator molecular changes that could be exploited in the clinic.

导致携带 BRCA1 或 BRCA2 突变的女性患乳腺癌的最初分子和细胞事件尚不清楚。我们将使用最先进的基因组工具（单细胞 RNA 测序和全基因组测序）来确定癌症在缺乏正常 BRCA 基因的情况下是如何开始的。来自癌前乳腺组织的干细胞和子细胞的单细胞基因组分析将用于识别可在临床中利用的早期指标分子变化。