Equipment for electron microscope image capture

一个由来自三个学科(生物学、化学和心理学)的8名Dalhousie电子显微镜专家组成的小组代表更大的用户群体，为继续运行先进的电子显微镜寻找设备。该显微镜是达尔豪西理工学院科学成像套件的核心设备。该设备包括两件物品：一台新的高分辨率数码相机，以及一台新的计算机和运行显微镜和相机的软件。对于取代老化和不可靠的嵌入式计算机来说，计算机是必不可少的，没有计算机，显微镜和相机就无法工作。这款相机需要取代分辨率和灵敏度较低的过时相机，这些相机无法捕捉到我们许多应用程序所需的足够高质量的图像。显微镜本身就是一种完美的电子光学仪器，寿命很长，但不能可靠或有效地操作来捕获图像。执行这些功能需要两个附加系统的设备，即照相机、计算机和相关软件。通过计算机和数字图像捕获技术的重大改进，预计更换部件的使用寿命将比它们计划更换的部件的使用寿命长得多。升级将支持的科学项目分布在不同的领域。申请者包括：果蝇的神经生物学、卤虫的细胞生物学、自由生活原生动物生物超微结构的进化方面、植物叶片中的细胞程序性死亡、视网膜神经元的突触发生、蛋白质泛素化在植物发育中的调节作用、治疗阿尔茨海默病的药物设计以及纳米颗粒材料科学。我们的共同申请者和用户代表了成熟的工人和年轻的同事，他们的计划将支持显微镜未来的操作。我们案例的优势取决于庞大的用户群体，他们的计划的卓越，这些提供的HQP培训的广度和深度，我们需求的紧迫性，替代方案的缺乏，以及投资于一个已经运行良好的系统的价值。

A group of eight Dalhousie electron microscopists from three disciplines (Biology, Chemistry and Psychology) seeks equipment for the continued operation of an advanced transmission electron microscope, on behalf of a larger group of users. The microscope is the core facility of the Science Faculty's Scientific Imaging Suite at Dalhousie. The equipment comprises two items: a new high-resolution digital camera, and a new computer and software to run both the microscope and camera. The computer is essential to replace an aging and unreliable embedded computer, without which the microscope and camera cannot function. The camera is needed to replace an outdated camera with inferior resolution and poor sensitivity that cannot capture images of sufficient quality for many of our applications. The microscope itself functions perfectly as an electron optical instrument, has a long life expectancy, but does not operate reliably or effectively to capture images. The equipment for both additional systems, i.e. the camera, computer and associated software, is needed to perform these functions. Through major improvements in both computer and digital image capture technologies, the useful life of the replacement items is predicted to be much longer than those of the components they are scheduled to replace. The scientific programs that would be supported by the upgrade are in diverse areas. Those of the applicants include: the neurobiology of Drosophila, the cell biology of Artemia, evolutionary aspects of biological ultrastructure in free-living protozoa, programmed cell death in plant leaves, synaptogenesis in retinal neurons, the regulatory role of protein ubiquitination in plant development, drug design against Alzheimer's disease, and nanoparticle materials science. Our co-applicants and users represent established workers and younger colleagues whose programs will sustain the future operation of the microscope. The strength of our case relies on the large group of users, the excellence of their programs, the breadth and depth of training these offer HQP, the urgency of our need, the lack of alternatives, and the value of investing in a system that is already working well.