Ultra-low temperature freezer and dry ice maker

超低温冰箱和干冰机

• 批准号: 390046-2010
• 负责人: Meinertzhagen, Ian
• 金额: $ 1.06万
• 依托单位: Dalhousie University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=443091
• 关键词: Ultra; low; temperature; freezer; dry

We are a group of behavioural neuroscientists who work on mammalian, avian and insect model systems, using a range of methods that require us to store samples at ultra-low temperatures (-80oC). Our studies on a range of nervous systems and tissues require us to generate and maintain tissue and tissue extract samples, reagents, and antibodies. These are all highly vulnerable and they are also very expensive, either to purchase or because we have invested a large amount of time or other human resources in collecting them. All need to be stored at ultra-low temperatures. A -80oC machine formerly available to us is no longer available, and we have only temporary access to an existing freezer belonging to a colleague who needs it for her own program. This is the only machine in our department. The replacement we now seek is therefore urgent both because we lack sufficient storage space at this temperature for all our needs, and because if the one working instrument, to which we have only short-term access, were ever to break down, we would lack any access to ultralow temperatures whatsoever. We are not able to transport frozen samples to nearby departments or buildings. We also need access to a dry-ice (solid CO2) machine to freeze specimens rapidly prior to storage. This cryogenic duo of equipment will support the research programs of four co-applicants and their undergraduate, graduate and postdoctoral trainees, at different career stages, whose programs converge on the neural mechanisms of behaviour and immunity.

我们是一群行为神经科学家，他们研究哺乳动物、鸟类和昆虫模型系统，使用一系列方法，要求我们在超低温（-80 ° C）下储存样品。 我们对一系列神经系统和组织的研究需要我们生成和维护组织和组织提取物样本、试剂和抗体。 这些都是非常脆弱的，它们也非常昂贵，无论是购买还是因为我们投入了大量的时间或其他人力资源来收集它们。 都需要在超低温下储存。 我们以前可用的-80 ℃机器已经不再可用，我们只能暂时使用一个现有的冷冻机，该冷冻机属于一位同事，她需要用于自己的项目。 这是我们部门唯一的机器。 因此，我们现在寻求的替代品是紧迫的，因为我们在这个温度下缺乏足够的存储空间来满足我们的所有需求，而且如果我们只能短期使用的一个工作仪器发生故障，我们将无法获得任何超低温。 我们无法将冷冻样本运送到附近的部门或建筑物。 我们还需要使用干冰（固体CO2）机，以便在储存前快速冷冻标本。 这两个低温设备将支持四个共同申请人及其本科生，研究生和博士后学员的研究计划，在不同的职业阶段，他们的计划集中在行为和免疫的神经机制。