Field survey of p53-gene sequence variations in Mytilus trossulus and assessment of other potential p53-gene isoforms

紫贻贝 p53 基因序列变异的现场调查和其他潜在 p53 基因亚型的评估

• 批准号: 364751-2008
• 负责人: Baldwin, Susan
• 金额: $ 3.16万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=406333
• 关键词: Field; survey; p53; gene; sequence

Canadian governments and municipalities are concerned about potentially harmful effects on the marine ecosystem caused by human activities. For example, in the lower mainland Metro Vancouver region, municipal wastewater is discharged, after treatment, at various locations along the coast. To help in addressing whether there are any changes in the health of ecosystems near to these effluent discharges, we are participating in an effort to test the use of mussels as sentinel species in so-called environmental effects monitoring (EEM) programs. In these programs, besides the basic physiological parameters such as size and reproductive development, the prevalence and intensity of various diseases are measured. For example, the local Pacific North West Coast Bay Mussel, M. trossulus, is susceptible to a leukemia-like disease called haemic neoplasia (HN), which has some similarities to a human cancer, Burkitts lymphoma. Breakouts of this disease have been known to wipe out entire populations. Therefore it is important to monitor incidences of HN regularly and we are involved in devising novel ways of doing this, while at the same time learning about some of the mechanisms of the disease. To enhance the ability of EEM programs to predict potential deleterious effects early, before irreversible ecosystem changes occur, we are specifically investigating the use of genes as biomarkers of HN. Our focus is on the tumour-suppressor p53-like genes since we have found associations between HN and expression of sequence variations of these genes. Therefore we propose that by monitoring these genes we may be able to predict HN outbreaks in mussel populations. Thus, the purpose of our current work is to test this hypothesis by monitoring p53 genes and HN in mussel populations on two beaches located in the Metro Vancouver area to see if there are any common trends between the gene sequences and the disease.

加拿大政府和市政当局对人类活动对海洋生态系统造成的潜在有害影响感到关切。 例如，在低大陆的大都市温哥华地区，城市废水在处理后排放到沿着的各个位置。 为了帮助解决这些污水排放附近生态系统的健康是否发生任何变化，我们正在参与一项努力，以测试贻贝作为所谓的环境影响监测（EEM）计划中的哨兵物种的使用。 在这些方案中，除了测量体型和生殖发育等基本生理参数外，还测量各种疾病的患病率和严重程度。 例如，当地的太平洋西北海岸湾贻贝，M。trossulus，易患白血病样疾病，称为血液瘤（HN），它与人类癌症伯基特淋巴瘤有一些相似之处。 据了解，这种疾病的爆发会使整个人口灭绝。 因此，定期监测HN的发病率是很重要的，我们参与了设计新的方法来做到这一点，同时了解疾病的一些机制。 为了提高EEM项目在不可逆的生态系统变化发生之前早期预测潜在有害影响的能力，我们正在专门研究使用基因作为HN的生物标志物。 我们的重点是肿瘤抑制p53样基因，因为我们已经发现HN和这些基因的序列变异的表达之间的关联。 因此，我们建议，通过监测这些基因，我们可能能够预测在贻贝种群的HN爆发。 因此，我们目前的工作的目的是通过监测位于大温哥华地区的两个海滩上的贻贝种群中的p53基因和HN来测试这一假设，看看基因序列和疾病之间是否有任何共同的趋势。