Refrigerated tabletop centrifuge

冷冻台式离心机

• 批准号: 374999-2009
• 负责人: Schulte, Patricia
• 金额: $ 1.06万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=412057
• 关键词: Refrigerated; tabletop; centrifuge

This proposal requests funds to replace a 9 year old refrigerated benchtop centrifuge that is broken and cannot be repaired. Refrigerated centrifuges are a core piece of laboratory equipment for all molecular biology and biochemistry laboratories and would be used daily by the research trainees in my research group, and by at least 6 other users in the Department of Zoology at the University of British Columbia. This centrifuge is an integral piece of equipment that will support research on the ways that fish adapt to the temperature of their environment. Temperature is known to be an important factor influencing the distribution and abundance of fish. Despite several decades of study, however, we still do not have a complete understanding of how fish respond to changes in environmental temperature during their lifetimes, or how adaptation to environmental change occurs over evolutionary time. In our current research we are testing the hypothesis that the increased metabolism required for adaptation to low temperatures comes at a cost of increased sensitivity to high temperatures, thus imposing a tradeoff between high metabolic rate and high thermal tolerance. We are also studying the molecular and biochemical basis of this effect. This research will provide an understanding of the physiological and molecular mechanisms underlying responses to temperature changes on both short and long time scales. This understanding will be particularly critical given the likely effects of global climate change.

这项提案要求提供资金，以更换一台已有9年历史的冷藏台式离心机，该离心机已损坏，无法修复。冷藏离心机是所有分子生物学和生物化学实验室的核心实验室设备，将由我的研究小组的研究实习生和不列颠哥伦比亚大学动物系的至少6名其他用户每天使用。这台离心机是一个不可或缺的设备，将支持鱼类适应环境温度的方式的研究。众所周知，温度是影响鱼类分布和丰度的重要因素。然而，尽管经过了几十年的研究，我们仍然没有完全了解鱼类在其一生中如何对环境温度的变化做出反应，或者如何在进化过程中适应环境变化。在我们目前的研究中，我们正在测试一种假设，即适应低温所需的新陈代谢增加是以增加对高温的敏感性为代价的，从而在高代谢率和高耐热性之间进行权衡。我们还在研究这种效应的分子和生化基础。这项研究将提供对短时间尺度和长时间尺度上对温度变化的潜在反应的生理和分子机制的理解。考虑到全球气候变化可能产生的影响，这一理解将特别关键。