HPLC sytem for analysis of lipids, carbohydrates and other bioactives

一个高效液相色谱（HPLC）系统被要求支持六名教师和他们的研究团队在达尔豪西的计划。所要求的配置包括蒸发光散射检测器（ELSD），该检测器被称为通用检测器，因为其能够检测所有分子，而无论其结构或电荷如何。它特别适用于无法用典型配置的仪器检测的化合物，如脂质。该HPLC的主要应用将是在脂质的分析中，特别是在完整脂质结构的表征和分离中。这种脂质分析将支持许多领域的研究，包括食物网的阐明，调查物理性质和结构之间的关系，并确定替代燃料来源。它还将在确定从动物和鱼类加工废物中回收的一系列生物活性物质方面发挥重要作用，包括脂溶性营养品，酶和胶原蛋白。HPLC系统还将用于营养物质的分析，以研究营养胁迫对细菌（特别是李斯特菌）存活的影响。最后，发酵和生物修复研究中的碳水化合物和碳氢化合物水平将使用该设备确定。因此，这里要求的HPLC系统将使六个申请人的不同研究项目取得重大进展。

A high performance liquid chromatography (HPLC) system is requested to support the programs of six faculty members and their research teams at Dalhousie. The requested configuration incorporates an evaporative light scattering detector (ELSD) which is known as a universal detector because of its ability to detect all molecules regardless of structure or charge. It is particularly appropriate for compounds such as lipids that cannot be detected with the typically configured instruments. The primary application of this HPLC will be in the analysis of lipids, specifically in the characterization and separation of intact lipid structures. Such lipid analysis will support research in a number of areas, including food web elucidation, investigating relationships between physical properties and structure, and identifying alternative fuel sources. It will also be essential in determining a range of bioactives recovered from animal and fish processing waste, including lipid-soluble nutraceuticals, enzymes and collagen. The HPLC system will also be used in the analysis of nutrients to investigate the impact of nutrient stress on survival of bacteria, specifically Listeria sp. Lastly, carbohydrate and hydrocarbon levels in fermentation and bioremediation studies will be determined using this equipment. Thus, the HPLC system requested here will enable significant advancements to be made in the diverse research programs of the six applicants.