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Work station for microplate analysis

微孔板分析工作站

基本信息

批准号：
375483-2009
负责人：
Grenier, Daniel
金额：
$ 2.57万
依托单位：
Université Laval
依托单位国家：
加拿大
项目类别：
Research Tools and Instruments - Category 1 (<$150,000)
财政年份：
2008
资助国家：
加拿大
起止时间：
2008-01-01 至 2009-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=412157
关键词：
Work station microplate analysis

项目摘要

My research program focuses on the host-pathogen interactions and involves Streptococcus suis (causative agent of meningitis and endocarditis in swine) and Porphyromonas gingivalis (causative of chronic periodontitis in humans). We are interested in demonstrating how the pathogens interact with host cells (endothelial cells, macrophages, etc) to colonize tissues, to resist the immune system, to amplify the inflammatory reaction and to cause tissue damage. Results from this research are aimed to provide new insights into the mechanisms by which these pathogens cause infections. This information will help for the development of effective means for controlling and/or preventing these infections. The requested work station for microplate analysis, a microplate washer and a microplate reader, will be mainly used to conduct immunoassays to quantify host-derived biomarkers (cytokines, chemokines, matrix metalloproteinases, etc) following stimulation of mammalian cells with bacteria and their products. The microplate reader will also be used to monitor biofilm formation by the swine pathogen S. suis. Finally, proteinase production and activity by host and bacterial cells will be quantified by the microplate reader using chromogenic substrates. The obtention of this grant will expand and improve student access to versatile and up-to-date analytical equipment.

我的研究项目侧重于宿主与病原体的相互作用，涉及猪链球菌（猪脑膜炎和心内膜炎的病原体）和牙龈卟啉单胞菌（人类慢性牙周炎的病原体）。我们感兴趣的是证明病原体如何与宿主细胞（内皮细胞、巨噬细胞等）相互作用以定殖组织、抵抗免疫系统、放大炎症反应并造成组织损伤。这项研究的结果旨在为这些病原体引起感染的机制提供新的见解。该信息将有助于开发控制和/或预防这些感染的有效方法。所需的微孔板分析工作站、微孔板清洗机和酶标仪将主要用于在用细菌及其产物刺激哺乳动物细胞后进行免疫测定，以量化宿主衍生的生物标志物（细胞因子、趋化因子、基质金属蛋白酶等）。酶标仪还将用于监测猪病原体猪链球菌的生物膜形成。最后，宿主和细菌细胞的蛋白酶产生和活性将通过酶标仪使用显色底物进行定量。获得这笔资助将扩大和改善学生获得多功能和最新分析设备的机会。