Laser-scanning confocal microscope adaptation for multi-user

适用于多用户的激光扫描共焦显微镜

基本信息

  • 批准号:
    375741-2009
  • 负责人:
  • 金额:
    $ 3.75万
  • 依托单位:
  • 依托单位国家:
    加拿大
  • 项目类别:
    Research Tools and Instruments - Category 1 (<$150,000)
  • 财政年份:
    2008
  • 资助国家:
    加拿大
  • 起止时间:
    2008-01-01 至 2009-12-31
  • 项目状态:
    已结题

项目摘要

This proposal requests funding for accessories that will allow the adaptation of existing equipment to create a multi-user laser scanning confocal microscope platform for the analysis of model organism development. The combined applicant laboratories study a broad range of fundamental questions relating to the processes of how cells and tissues respond to signals during development, with applications extending from the study of the establishment of cell polarity, to neural, retinal and muscle development and genetic control of neural tube defects, to systems biology approaches to the regulation of metabolism. The unifying theme of these research programs is the use of model organisms, which are amenable to the study of proteins at subcellular levels within the context of the intact, living animal, and the resulting effect of changes in these proteins on the dynamic structure of the tissues in which they are expressed. The key piece of equipment necessary for these studies is a laser-scanning confocal microscope with the capability to both image thicker specimens and to do time-lapse studies; a capability that is not currently available at the University. The basic microscope is being provided to the group by one of the co-applicants. We are requesting funds for accessories to allow this capability, to greatly increase the flexibility for a larger variety of research applications, and to facilitate multi-user image acquisition, processing and storage. These accessories include a high resolution digital camera, microscope objectives with a long working-distance, optical filters allowing the use of a wider set of fluorescent markers and a computer workstation with data storage and data security capabilities. By modifying existing equipment, we can achieve this goal at a fraction of the usual cost.
该提案要求为配件提供资金,以便改造现有设备,建立一个多用户激光扫描共聚焦显微镜平台,用于分析模式生物的发育。联合申请人实验室研究与细胞和组织在发育过程中如何响应信号的过程有关的广泛的基本问题,应用范围从细胞极性的建立研究,到神经,视网膜和肌肉发育以及神经管缺陷的遗传控制,再到调节代谢的系统生物学方法。这些研究项目的统一主题是使用模式生物,这些模式生物适合于在完整的活动物的背景下在亚细胞水平上研究蛋白质,以及这些蛋白质的变化对表达它们的组织的动态结构的影响。这些研究所需的关键设备是一台激光扫描共聚焦显微镜,它能够对较厚的标本进行成像并进行延时研究;这是该大学目前所没有的能力。共同申请人之一正在向小组提供基本显微镜。 我们正在申请资金,用于附件,以实现这一能力,大大提高更广泛的研究应用的灵活性,并促进多用户图像采集,处理和存储。 这些配件包括高分辨率数码相机、工作距离长的显微镜物镜、允许使用更广泛荧光标记的滤光片以及具有数据存储和数据安全功能的计算机工作站。 通过改造现有设备,我们可以以通常成本的一小部分实现这一目标。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Pilgrim, David其他文献

The myosin chaperone UNC45B is involved in lens development and autosomal dominant juvenile cataract
  • DOI:
    10.1038/ejhg.2014.21
  • 发表时间:
    2014-11-01
  • 期刊:
  • 影响因子:
    5.2
  • 作者:
    Hansen, Lars;Comyn, Sophie;Pilgrim, David
  • 通讯作者:
    Pilgrim, David
The British welfare state and mental health problems: the continuing relevance of the work of Claus Offe
  • DOI:
    10.1111/j.1467-9566.2011.01447.x
  • 发表时间:
    2012-09-01
  • 期刊:
  • 影响因子:
    2.9
  • 作者:
    Pilgrim, David
  • 通讯作者:
    Pilgrim, David
Innovation in mental health services: what are the key components of success?
  • DOI:
    10.1186/1748-5908-6-120
  • 发表时间:
    2011-10-26
  • 期刊:
  • 影响因子:
    7.2
  • 作者:
    Brooks, Helen;Pilgrim, David;Rogers, Anne
  • 通讯作者:
    Rogers, Anne
Critical realism as a continuing resource for biological research: the illustrative case study of biting midges and their symbiotic bacteria
  • DOI:
    10.1080/14767430.2021.1877490
  • 发表时间:
    2021-02-02
  • 期刊:
  • 影响因子:
    2.6
  • 作者:
    Pilgrim, Jack;Pilgrim, David
  • 通讯作者:
    Pilgrim, David
'Recovery' and current mental health policy
  • DOI:
    10.1177/1742395308097863
  • 发表时间:
    2008-12-01
  • 期刊:
  • 影响因子:
    1.3
  • 作者:
    Pilgrim, David
  • 通讯作者:
    Pilgrim, David

Pilgrim, David的其他文献

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{{ truncateString('Pilgrim, David', 18)}}的其他基金

Molecular pathways maintaining homeostasis in the vertebrate muscle sarcomere
维持脊椎动物肌肉肌节稳态的分子途径
  • 批准号:
    RGPIN-2018-06684
  • 财政年份:
    2022
  • 资助金额:
    $ 3.75万
  • 项目类别:
    Discovery Grants Program - Individual
Molecular pathways maintaining homeostasis in the vertebrate muscle sarcomere
维持脊椎动物肌肉肌节稳态的分子途径
  • 批准号:
    RGPIN-2018-06684
  • 财政年份:
    2021
  • 资助金额:
    $ 3.75万
  • 项目类别:
    Discovery Grants Program - Individual
Molecular pathways maintaining homeostasis in the vertebrate muscle sarcomere
维持脊椎动物肌肉肌节稳态的分子途径
  • 批准号:
    RGPIN-2018-06684
  • 财政年份:
    2020
  • 资助金额:
    $ 3.75万
  • 项目类别:
    Discovery Grants Program - Individual
Molecular pathways maintaining homeostasis in the vertebrate muscle sarcomere
维持脊椎动物肌肉肌节稳态的分子途径
  • 批准号:
    RGPIN-2018-06684
  • 财政年份:
    2019
  • 资助金额:
    $ 3.75万
  • 项目类别:
    Discovery Grants Program - Individual
Molecular pathways maintaining homeostasis in the vertebrate muscle sarcomere
维持脊椎动物肌肉肌节稳态的分子途径
  • 批准号:
    RGPIN-2018-06684
  • 财政年份:
    2018
  • 资助金额:
    $ 3.75万
  • 项目类别:
    Discovery Grants Program - Individual
Muscle Protein Complex Assembly and Maintenance
肌肉蛋白复合物的组装和维护
  • 批准号:
    RGPIN-2017-05268
  • 财政年份:
    2017
  • 资助金额:
    $ 3.75万
  • 项目类别:
    Discovery Grants Program - Individual
Cell biology of a specialized epithelium: regulation of the cellularizing C. elegans germline
特殊上皮细胞的细胞生物学:细胞化秀丽隐杆线虫种系的调节
  • 批准号:
    105466-2012
  • 财政年份:
    2016
  • 资助金额:
    $ 3.75万
  • 项目类别:
    Discovery Grants Program - Individual
Cell biology of a specialized epithelium: regulation of the cellularizing C. elegans germline
特殊上皮细胞的细胞生物学:细胞化秀丽隐杆线虫种系的调节
  • 批准号:
    105466-2012
  • 财政年份:
    2015
  • 资助金额:
    $ 3.75万
  • 项目类别:
    Discovery Grants Program - Individual
Cell biology of a specialized epithelium: regulation of the cellularizing C. elegans germline
特殊上皮细胞的细胞生物学:细胞化秀丽隐杆线虫种系的调节
  • 批准号:
    105466-2012
  • 财政年份:
    2014
  • 资助金额:
    $ 3.75万
  • 项目类别:
    Discovery Grants Program - Individual
Cell biology of a specialized epithelium: regulation of the cellularizing C. elegans germline
特殊上皮细胞的细胞生物学:细胞化秀丽隐杆线虫种系的调节
  • 批准号:
    105466-2012
  • 财政年份:
    2013
  • 资助金额:
    $ 3.75万
  • 项目类别:
    Discovery Grants Program - Individual

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