Gel documentation and fluorescent imaging workstation for modular and genetic studies

用于模块化和遗传研究的凝胶记录和荧光成像工作站

• 批准号: 375911-2009
• 负责人: Chen, JinGui
• 金额: $ 1.23万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=412259
• 关键词: Gel; documentation; fluorescent; imaging; workstation

Arabidopsis is a model organism for agricultural biotechnology, providing the opportunity to get key insights into the way that gene function can affect crop improvement. With the completion of the Arabidopsis genome sequencing project, the current challenge is the determination of gene function. A complete understanding of a gene's function relies extensively on molecular and genetic studies. The proposed Gel Documentation and Fluorescent Imaging Workstation is one of the key components of the PI's gene function determination platform. The proposed Workstation facilitates nucleic acid detection, protein detection, and Petri-dish/microplate imaging through fluorescent and colorimetric imaging. It offers imaging acquisition, analysis and enhancement. The proposed Workstation is essential for molecular and genetic studies. Funds ($16,950) are requested to purchase a Gel Documentation and Fluorescent Imaging Workstation devoted to the research program described in the proposal entitled "Molecular mechanisms of the action of Arabidopsis RACK1, an ortholog of receptor for activated protein kinase C", funded by the NSERC Discovery Grant.

拟南芥是农业生物技术的模式生物，为深入了解基因功能如何影响作物改良提供了机会。 随着拟南芥基因组测序计划的完成，目前的挑战是确定基因功能。 对基因功能的完整理解广泛依赖于分子和遗传学研究。 建议的凝胶文档和荧光成像工作站是PI的基因功能测定平台的关键组成部分之一。 拟议的工作站通过荧光和比色成像促进核酸检测、蛋白质检测和培养皿/微孔板成像。 它提供成像采集、分析和增强。 拟议的工作站是必不可少的分子和遗传研究。 资金（16，950美元）用于购买凝胶文件和荧光成像工作站，专门用于题为“拟南芥RACK 1的分子作用机制，活化蛋白激酶C受体的直系同源物”的提案中描述的研究计划，由NSERC发现赠款资助。