Gel documentation and fluorescent imaging workstation for modular and genetic studies

拟南芥是农业生物技术的模式生物，为深入了解基因功能如何影响作物改良提供了机会。随着拟南芥基因组测序计划的完成，目前面临的挑战是基因功能的确定。对基因功能的完整理解广泛依赖于分子和遗传学研究。拟议的凝胶文档和荧光成像工作站是PI基因功能确定平台的关键组件之一。拟议的工作站通过荧光和比色成像促进了核酸检测、蛋白质检测和培养皿/微板成像。它提供图像采集、分析和增强功能。拟议的工作站对分子和遗传学研究是必不可少的。需要资金(16,950美元)，用于购买凝胶文档和荧光成像工作站，该工作站专门用于由NSERC发现赠款资助的题为“拟南芥RACK1的作用分子机制”的提案中描述的研究项目，拟南芥RACK1是活化蛋白激酶C受体的同源受体。

Arabidopsis is a model organism for agricultural biotechnology, providing the opportunity to get key insights into the way that gene function can affect crop improvement. With the completion of the Arabidopsis genome sequencing project, the current challenge is the determination of gene function. A complete understanding of a gene's function relies extensively on molecular and genetic studies. The proposed Gel Documentation and Fluorescent Imaging Workstation is one of the key components of the PI's gene function determination platform. The proposed Workstation facilitates nucleic acid detection, protein detection, and Petri-dish/microplate imaging through fluorescent and colorimetric imaging. It offers imaging acquisition, analysis and enhancement. The proposed Workstation is essential for molecular and genetic studies. Funds ($16,950) are requested to purchase a Gel Documentation and Fluorescent Imaging Workstation devoted to the research program described in the proposal entitled "Molecular mechanisms of the action of Arabidopsis RACK1, an ortholog of receptor for activated protein kinase C", funded by the NSERC Discovery Grant.