Plant growth chamber for arabidopsis research

用于拟南芥研究的植物生长室

• 批准号: 359247-2008
• 负责人: Chen, JinGui
• 金额: $ 2.08万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=361352
• 关键词: Plant; growth; chamber; arabidopsis; research

Arabidopsis is a model organism for agricultural biotechnology, providing the opportunity to get key insights into the way that gene function can affect crop improvement. With the completion of the Arabidopsis genome sequencing project, the current challenge is the determination of gene function. A complete understanding of a gene's function relies on the phenotypic analysis of the mutant alleles and transgenic lines for the gene of interest. However, the manifestation of phenotype relies on the growth conditions. Subtle phenotypes are often missed in less desirable conditions. Therefore, a controllable growth environment is essential for the phenotypic analysis of Arabidopsis mutants and transgenic lines. Because of its small size, rosette growth habit, and short life cycle, an optimal growth of Arabidopsis can be achieved in a growth chamber specifically designed for this model laboratory plant.                The proposed Arabidopsis growth chamber will be one of the key components for the PI's gene function determination platform. This growth chamber is specific designed for Arabidopsis growth, providing controlled environments for phenotypic analysis and genetic screen. The acquisition of such an Arabidopsis growth chamber would permit the observation and record of the phenotypes at many developmental stages, from seedlings to mature plants. Funds ($28,501) are requested to purchase an Arabidopsis growth chamber devoted to the research program described in the proposal entitled "Molecular mechanisms of the action of Arabidopsis RACK1, an ortholog of receptor for activated protein kinase C", funded by the NSERC Discovery Grants.

拟南芥是农业生物技术的模式生物，为深入了解基因功能如何影响作物改良提供了机会。随着拟南芥基因组测序计划的完成，目前的挑战是确定基因功能。对基因功能的完整理解依赖于对目的基因的突变等位基因和转基因株系的表型分析。然而，表型的表现依赖于生长条件。微妙的表型往往在不太理想的条件下错过。因此，一个可控的生长环境对于拟南芥突变体和转基因株系的表型分析至关重要。由于其体积小，莲座状生长习性和短生命周期，拟南芥的最佳生长可以在专门为该模型实验室植物设计的生长室中实现。 建议的拟南芥生长室将是PI的基因功能测定平台的关键组成部分之一。该生长室是专为拟南芥生长而设计的，为表型分析和遗传筛选提供受控环境。获得这样的拟南芥生长室将允许在许多发育阶段，从幼苗到成熟植物的表型的观察和记录。资金（28，501美元）被要求购买一个拟南芥生长室，专门用于题为“拟南芥RACK 1，活化蛋白激酶C受体的直系同源物”的提案中描述的研究计划，由NSERC发现赠款资助。