人类阿尔茨海默病大脑的空间单细胞分析-猫眼课题宝

人类阿尔茨海默病大脑的空间单细胞分析

批准号：

10822988

负责人：

Kirsten Frieda

金额：

$122.93万

依托单位：

SPATIAL GENOMICS, INC.

依托单位国家：

美国

项目类别：

财政年份：

2023

资助国家：

美国

项目状态：

未结题

起止时间：

2023-09-21 至 2025-05-31

来源：

https://reporter.nih.gov/project-details/10822988

关键词：

Address Alzheimer&apos s Disease Alzheimer&apos s disease brain Animals Antibodies Area Autopsy Bar Codes Biological Assay Brain Cell Communication Cells Collaborations Collection Complex Computer software Data Data Set Devices Disease Disease Progression Environment Epigenetic Process Fluorescent in Situ Hybridization Gene Expression Gene Expression Profiling Gene Order Genes Genomics Geography Gliosis Goals Human Image Immunofluorescence Immunologic In Situ In Situ Hybridization Life Maps Measurement Measures Messenger RNA Methods Microglia Molecular Morphology Mus Neighborhoods Neurofibrillary Tangles Neurons Noise Pathology Population Procedures Process Protein Analysis Proteins Protocols documentation RNA RNA amplification RNA analysis Reaction Reagent Research Personnel Resolution Sampling Scheme Senile Plaques Signal Transduction Slice Synapses System Technology Tissues Vascular Diseases brain tissue cell type commercialization experience experimental study genomic tools human disease instrument multimodality multiple omics neuron loss neuropathology preservation protein TDP-43 protein biomarkers protein expression protein profiling single cell analysis single molecule transcriptome

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英文摘要

SUMMARY The major goal of this project is to study human Alzheimer’s Disease (AD) brains using the Spatial Genomics GenePS instrument for automated large scale gene expression analysis at single cell resolution. At Spatial Genomics, we are commercializing the sequential fluorescence in situ hybridization (seqFISH) technology (Eng et al, 2019) that allows thousands of genes to be imaged directly in brain samples. We have experience working with human brains with our seqFISH technology and will hone the technology for use in human brains. Using the seqFISH method, we will identify changes in cell types, spatial organization of groups of cells, and signaling interactions between neighboring cells in the human AD brain.

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