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Spatial single cell analysis of the human Alzheimer's Disease brain

人类阿尔茨海默病大脑的空间单细胞分析

基本信息

批准号：
10822988
负责人：
Kirsten Frieda
金额：
$ 122.93万
依托单位：
SPATIAL GENOMICS, INC.
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-09-21 至 2025-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10822988
关键词：
Address Alzheimer&apos s Disease Alzheimer&apos s disease brain Animals Antibodies Area Autopsy Bar Codes Biological Assay Brain Cell Communication Cells Collaborations Collection Complex Computer software Data Data Set Devices Disease Disease Progression Environment Epigenetic Process Fluorescent in Situ Hybridization Gene Expression Gene Expression Profiling Gene Order Genes Genomics Geography Gliosis Goals Human Image Immunofluorescence Immunologic In Situ In Situ Hybridization Life Maps Measurement Measures Messenger RNA Methods Microglia Molecular Morphology Mus Neighborhoods Neurofibrillary Tangles Neurons Noise Pathology Population Procedures Process Protein Analysis Proteins Protocols documentation RNA RNA amplification RNA analysis Reaction Reagent Research Personnel Resolution Sampling Scheme Senile Plaques Signal Transduction Slice Synapses System Technology Tissues Vascular Diseases brain tissue cell type commercialization experience experimental study genomic tools human disease instrument multimodality multiple omics neuron loss neuropathology preservation protein TDP-43 protein biomarkers protein expression protein profiling single cell analysis single molecule transcriptome

项目摘要

SUMMARY The major goal of this project is to study human Alzheimer’s Disease (AD) brains using the Spatial Genomics GenePS instrument for automated large scale gene expression analysis at single cell resolution. At Spatial Genomics, we are commercializing the sequential fluorescence in situ hybridization (seqFISH) technology (Eng et al, 2019) that allows thousands of genes to be imaged directly in brain samples. We have experience working with human brains with our seqFISH technology and will hone the technology for use in human brains. Using the seqFISH method, we will identify changes in cell types, spatial organization of groups of cells, and signaling interactions between neighboring cells in the human AD brain.

总结该项目的主要目标是利用人类大脑中的神经元研究阿尔茨海默病（AD）的大脑。空间基因组学GenePS仪器用于自动化大规模基因表达分析单细胞分辨率。在Spatial Genomics，我们正在将连续荧光原位杂交（seqFISH）技术（Eng et al，2019）允许数千个基因直接在大脑样本中成像。我们有经验与人类的大脑工作与我们的 seqFISH技术，并将磨练该技术用于人类大脑。使用seqFISH 方法，我们将确定细胞类型的变化，细胞群的空间组织，人类AD大脑中相邻细胞之间的信号相互作用。