Skeletal muscle force modulation: length dependent factors

骨骼肌力调节：长度相关因素

• 批准号: 105674-2008
• 负责人: MacIntosh, Brian
• 金额: $ 1.6万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=421769
• 关键词: Skeletal; muscle; force; modulation; length

It is my goal to better understand the fundamental properties of muscles. Active force has been calculated by: peak force - passive force measured at the whole muscle length. We showed that passive force can decrease during a contraction and that active force could be correctly calculated if fiber bundle length is measured using sonomicrography: ultrasound is emitted from a crystal implanted at one end of the fiber bundle and detected by a crystal at the other end. I will investigate properties of muscle that may have previously been misinterpreted due to active force miscalculation. The anesthetized rat medial gastrocnemius muscle will be tested in situ. Individual motor units (defined as any motor neuron and all muscle fibers it innervates) will be activated alone, or in combination with another one with different sized motor units, and at several lengths. Passive force is greater at long lengths, so the miscalculation is greater. I believe the corrected method will give force of two motor units activated together similar to the sum of the two activated separately. There are several reports that lengthening contractions cause the relationship between length and active force to shift to the right. It has been suggested that the shift to longer lengths is caused by increased compliance, but this has not been directly assessed. I will measure compliance, using sonomicrography, and cause damage in muscle, by lengthening contractions or needle piercing and compare the contractile response (including compliance). It is hypothesized that a shift to the right will only be seen when passive force decreases due to the treatment, and active force is calculated in the traditional manner. The rightward shift will not be evident with the corrected method of calculating active force. Muscle responds to increased or decreased use by changing protein synthesis. It is thought that cell signalling mechanisms are sensitive to the force in the muscle, resulting in activation of some key signalling molecules. I propose to measure these, to see if active force, calculated in the correct way is a more sensitive predictor of this than the traditional method. The work that is proposed will advance our fundamental understanding of how muscles function.

我的目标是更好地了解肌肉的基本特性。 主动力的计算公式为：峰值力-在整个肌肉长度上测量的被动力。 我们表明，被动力可以减少收缩过程中，主动力可以正确计算，如果纤维束长度测量使用sonomicrography：超声波是从植入在纤维束的一端的晶体发射，并检测到晶体在另一端。 我将研究肌肉的性质，以前可能被误解，由于主动力计算错误。 将对麻醉的大鼠内侧腓肠肌进行原位测试。 单个运动单元（定义为任何运动神经元及其支配的所有肌肉纤维）将单独激活，或与具有不同大小运动单元的另一个运动单元组合，并以几种长度激活。 在较长的长度上，被动力更大，因此误判更大。 我相信修正后的方法将使两个运动单位一起激活的力量与两个单独激活的力量之和相似。 有几个报告说，延长收缩导致长度和主动力之间的关系向右移动。 有人认为，向更长长度的转变是由于依从性增加所致，但尚未直接评估。 我将使用声显微成像术测量顺应性，并通过延长收缩或针刺造成肌肉损伤，并比较收缩反应（包括顺应性）。 假设仅当被动力因治疗而降低时才会观察到向右偏移，并且以传统方式计算主动力。 用修正的计算主动力的方法，这种位移将不明显。 肌肉通过改变蛋白质合成来对增加或减少使用做出反应。 据认为，细胞信号机制对肌肉中的力敏感，导致一些关键信号分子的激活。 我建议测量这些，看看以正确的方式计算的主动力是否比传统方法更敏感。 这项工作将促进我们对肌肉功能的基本理解。