Technology development for proteome analysis incorporating separations and mass spectrometry

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Many significant advancements in biological science have followed technological advancements in analytical chemistry, which afford new opportunities for sample analysis. Proteomics is a perfect example of this, where advances in mass spectrometry, separation science, and computational analysis have permitted the comprehensive characterization of proteins. In doing so, one can essentially obtain a 'molecular' description of an organism, and therefore provide an improved understanding of biological pathways, assist in drug design, and also provide molecular fingerprints which identify a particular state of an organism (a.k.a. disease biomarkers). Current challenges in proteome analysis continue to evolve, as new technologies are created. What was once a problem of ionization and interpretation of mass spectrometry patterns, proteomics now focuses (among other aspects) on further increasing sample throughput to enable the analysis of clinically significant sample sizes. The characterization of protein modifications, quantitation of changing protein expression, and the validation of mass spectral data also continue to be topics of continued research in this field.