Revealing the expression, degradation and binding characteristics fo the glycosaminoglycans

揭示糖胺聚糖的表达、降解和结合特征

• 批准号: 312637-2008
• 负责人: Nitz, Mark
• 金额: $ 3.64万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=428325
• 关键词: Revealing; expression; degradation; binding; characteristics

Heparan sulfate (HS) is a linear polysaccharide that is ubiquitous in complex organisms. It is found in the space between cells and is proposed to orchestrate many biological events that coordinate organismal development and maintenance. HS has also been found to be a key player in many human diseases ranging from cancer to bacterial and viral infections. Despite the wide spread presence and importance of HS, very few tools are available to study its overall structure. Our research program is focused on developing new tools and techniques to facilitate HS research. We aim to generate conjugates that mimic the structure of HS. We will test the ability of these compounds to interact with a variety of HS binding proteins important in immune function. Understanding how these HS mimics function and comparing the findings to to those observed with the natural HS polymer will lead to a better understanding of HS and how diseases which involve HS may be controlled. We also aim to prepare fluorescent sensors able to report on the macromolecular structure of HS. Being able to follow changes in the structure of HS will lead to important findings as to how HS regulates biological function.

硫酸乙酰肝素（HS）是一种线性多糖，普遍存在于复杂生物体中。 它存在于细胞之间的空间，被认为是协调许多生物事件，协调有机体的发育和维护。 HS也被发现是许多人类疾病的关键参与者，从癌症到细菌和病毒感染。尽管HS的广泛存在和重要性，但很少有工具可用于研究其整体结构。我们的研究计划专注于开发新的工具和技术，以促进HS研究。我们的目标是产生模拟HS结构的缀合物。 我们将测试这些化合物与多种在免疫功能中重要的HS结合蛋白相互作用的能力。 了解这些HS模拟物如何发挥作用，并将这些发现与天然HS聚合物的观察结果进行比较，将有助于更好地了解HS以及如何控制涉及HS的疾病。 我们还旨在制备能够报告HS大分子结构的荧光传感器。 能够跟踪HS结构的变化将导致HS如何调节生物功能的重要发现。