New dimensions for mass cytometry and imaging mass cytometry

质谱流式分析和成像质谱流式分析的新维度

• 批准号: 505236-2016
• 负责人: Nitz, Mark
• 金额: $ 5.41万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=660990
• 关键词: New; dimensions; mass; cytometry; imaging

Biological systems are exceptionally complex. To understand the biochemical processes that influence diseases in whole organisms, information about all the cell types present and their activities is required. Mass cytometry is a new analysis method that enables the simultaneous quantification of a large number of parameters at the cellular level in heterogeneous cell populations. Using mass cytometry more information than ever before can be obtained from, for example, a single blood sample or biopsy sample. Previous methods were able to measure upwards of ten parameters at a time but mass cytometry has the potential to measure over 100. The key to realising the potential of mass cytometry is the availability of are the reagents it uses. Currently, we have developed chemistry to employ over 30 different mass cytometry reagents. We need to expand the repertoire of heavy isotopes that can be harnessed for MC reagents and this will in turn increase the amount and type of information that can be obtained from mass cytometry. In this proposal we aim to dramatically increase the elements that are available for mass cytometry experiments through the development of reagents that incorporate Te, Sn, Pd, Pt, Ru, Rh, In and Ag. This panel of elements should provide an additional 25-30 reagents when synthesised from isotopically enriched samples. Moreover, we aim to develop chemistry for a series of low molecular weight probes that can be used as general stains as well as to report on specific enzyme activities. These reagents will become commercial products sold by Fluidigm Canada to an ever growing international market. In addition the project will provide a fertile training ground for highly qualified personnel in the fields of chemistry and biotechnology.**

生物系统异常复杂。为了了解影响整个生物体疾病的生化过程，需要了解所有细胞类型及其活动的信息。质量细胞术是一种新的分析方法，能够在异质细胞群体的细胞水平上同时定量大量参数。使用细胞计数术可以获得比以往更多的信息，例如，从单个血液样本或活检样本。以前的方法能够一次测量10个以上的参数，但质量细胞术有可能测量100多个参数。实现细胞计数技术潜力的关键是它所使用的试剂的可用性。目前，我们已经开发了化学，使用超过30种不同的细胞计数试剂。我们需要扩大可用于MC试剂的重同位素库，这将反过来增加可从细胞计数法获得的信息的数量和类型。在本提案中，我们的目标是通过开发含有Te， Sn, Pd, Pt, Ru, Rh， In和Ag的试剂，大幅增加可用于细胞计数实验的元素。当从同位素富集的样品合成时，这组元素应提供额外的25-30种试剂。此外，我们的目标是开发一系列低分子量探针的化学反应，这些探针可以用作一般的染色剂，也可以报告特定的酶活性。这些试剂将成为Fluidigm加拿大公司的商业产品，销售到不断增长的国际市场。此外，该项目将为化学和生物技术领域的高素质人才提供肥沃的培训场地