Mass spectrometry and optical spectroscopy studies to characterize biological molecules in controlled micro-environments

质谱和光谱研究，用于表征受控微环境中的生物分子

• 批准号: 327206-2009
• 负责人: Jockusch, Rebecca
• 金额: $ 2.91万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=428706
• 关键词: Mass; spectrometry; optical; spectroscopy; studies

The correct functioning of biological molecules such as proteins is dependent on molecular properties including structure and stability. My research program is designed to provide a better understanding of the intrinsic properties of biological molecules and of how these properties are influenced by interactions with molecules in the local environment, such as metal ions, other proteins, small ligands and even the ever-present water molecules. The approach we take is to characterize biomolecules and biomolecular complexes in a highly-controlled gas-phase environment. This affords valuable simplification of complex biological systems. We first characterize intrinsic properties of the biomolecule when it is isolated in the gas phase. Then, we determine the effects of relevant molecular interactions by adding in pieces of the local molecular environment, making such as a small number of water molecules. Our experiments combine the analytically powerful techniques of mass spectrometry with an added dimension of laser spectroscopic data.

蛋白质等生物分子的正确功能取决于分子的性质，包括结构和稳定性。我的研究项目旨在更好地理解生物分子的内在特性，以及这些特性如何受到与局部环境中分子的相互作用的影响，例如金属离子、其他蛋白质、小配体，甚至是永远存在的水分子。我们采用的方法是在高度控制的气相环境中表征生物分子和生物分子复合物。这为复杂的生物系统提供了有价值的简化。当生物分子在气相分离时，我们首先表征其固有特性。然后，我们通过添加局部分子环境的片段来确定相关分子相互作用的影响，例如少量的水分子。我们的实验结合了强大的质谱分析技术和激光光谱数据的附加维度。