"Development, Characterization, and Application of Bio-analytical Methodologies Based on Optical and Mass Spectrometry"

质谱学(MS)是最强大的化学和生化分析技术之一，特别是与高效液相色谱(LC)等分离方法相结合时。我们正在研究大气压光电离(APPI)，这是一种用于连接LC和MS的电离方法。我们的研究旨在表征和改进APPI的性能，包括开发新型APPI器件。我们研究的另一个目标是开发一种实用的新方法来实现多肽和蛋白质的电子捕获解离(ECD)，适用于所有的电喷雾质谱仪。这项工作将建立在我们最近发明的源内大气压力ECD方法的基础上，该方法基于APPI技术。另一个目标是开发和演示在应用中使用AP-ECD的完整方法。这项工作很重要，因为它将为世界各地的研究人员提供具有成本效益的ECD技术，这是生物和生物医学研究中多肽和蛋白质分析的强大工具。

Mass spectrometry (MS) is one of the most powerful techniques for chemical and biochemical analysis, particularly when combined with a separation method such as liquid chromatography (LC). We are investigating atmospheric pressure photoionization (APPI), an ionization method for interfacing LC and MS. Our research aims to both characterize and improve the performance of APPI, including developing novel APPI devices. An additional objective of our research is to develop a practical new means of implementing electron capture dissociation (ECD) of peptides and proteins, suitable for use with all electrospray mass spectrometers. This work will build upon our recent invention of an in-source atmospheric pressure ECD method, based upon APPI technology. Another aim is to develop and demonstrate complete methods for using AP-ECD in applications. This work is important because it will provide researchers worldwide cost-effective access to ECD technology, a powerful tool for peptide and protein analysis in biological and biomedical research.