Multiplexed field-effect transistor nanobiosensors: Bioanotechnology for environmental monitoring of airborne pathogens and for homeland security

多重场效应晶体管纳米生物传感器：用于空气传播病原体环境监测和国土安全的生物纳米技术

• 批准号: 365059-2008
• 负责人: Leung, KamTong
• 金额: $ 13.72万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Strategic Projects - Group
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=434445
• 关键词: Multiplexed; field; effect; transistor; nanobiosensors

Fast response is the most effective measure to protect the public from infectious disease outbreaks (e.g., 2003 SARS in Toronto, and bird flu in recent years) and bioterrorism (e.g., 2001 anthrax attack in the United States), and to provide early detection of toxic pollutants in our environment and of microbial contamination of our food supply. The proposed project seeks to develop a novel multiplex sensor system that is based on nanoscale field-effect transistors (involving carbon nanotubes and transparent oxide nanowalls). By deploying these nanobiosensors in public places (hospitals, schools, airports, transit systems, sporting arenas, shopping malls), this nanobiosensor system offers fast, parallel detection of multiple airborne pathogens (including viruses, bacteria, and toxins) simultaneously, thereby providing an early warning system of any dangerous substances in the air that we breathe. Our study will specifically focus on developing the general methodologies for (a) the fabrication of this nanoscale electronic sensor system based on novel nanomaterials, and (b) biofunctionalization of the transistors with appropriate receptors to capture the target pathogens of interest as whole-organisms. These methods can be generalized and extended to develop the appropriate nanobiosensors to facilitate detection of most of the common airborne viruses and environmental toxins in a typical Canadian city like Toronto or Waterloo. The proposed project will take advantage of the high surface-area-to-volume ratio and the surface structures of carbon nanotubes and transparent oxide nanostructured materials to dramatically enhance the uptake of bioreceptors, which will in turn greatly increase the detection efficiency of the biosensors. By building an array of these nanobiosensors onto a single chip, we can increase the efficiency

快速反应是保护公众免受传染病爆发影响的最有效措施（例如，2003年多伦多的SARS，以及近年来的禽流感）和生物恐怖主义（例如，2001年美国炭疽袭击），并提供早期检测有毒污染物在我们的环境和微生物污染我们的食品供应。拟议的项目旨在开发一种新型的多路传感器系统，该系统基于纳米级场效应晶体管（包括碳纳米管和透明氧化物半导体）。通过将这些纳米生物传感器部署在公共场所（医院，学校，机场，交通系统，体育场，购物中心），这种纳米生物传感器系统可以同时快速，并行地检测多种空气传播的病原体（包括病毒，细菌和毒素），从而为我们呼吸的空气中的任何危险物质提供预警系统。我们的研究将特别侧重于开发的一般方法（a）这种纳米级电子传感器系统的制造基于新型纳米材料，和（B）生物功能化的晶体管与适当的受体，以捕获目标病原体的利益作为整个有机体。这些方法可以推广和扩展，以开发适当的纳米生物传感器，以促进检测大多数常见的空气传播的病毒和环境毒素在一个典型的加拿大城市，如多伦多或滑铁卢。该项目将利用碳纳米管和透明氧化物纳米结构材料的高表面积与体积比和表面结构，大大提高生物受体的吸收，从而大大提高生物传感器的检测效率。通过在单个芯片上构建这些纳米生物传感器阵列，我们可以提高效率