Acquisition of plant tissue culture chambers/Acquistion des chambres de culture des tissus végétaux

收购植物组织培养室/Acquistion des chambres deculture des tissus végétaux

• 批准号: 389593-2010
• 负责人: Moffett, Peter
• 金额: $ 2.27万
• 依托单位: Université de Sherbrooke
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=442961
• 关键词: Acquisition; plant; tissue; culture; chambers

We study the ability of plants to defend themselves against pathogens. In many cases, this disease resistance is conferred by specific disease resistance (R) genes, many of which have been cloned from solanaceous crop plants (potato, tomato, pepper). However, the molecular analysis of these genes is much easier in model plants such as tobacco. The generation of such model systems necessitates the ability to generate transgenic plants, which requires specialized sterile environments, including plant tissue culture chambers. The latter allow for precise control of temperature, light and humidity and are used for the regeneration of transgenic material. This material is in turn used either to establish experimental systems or to validate candidate genes and proteins for their involvement in plant defences. The information gleaned from these experimental systems can then be applied to the better use of R genes in their native context in crop plants.

我们研究植物防御病原体的能力。 在许多情况下，这种抗病性是由特异性抗病（R）基因赋予的，其中许多基因已从茄科作物植物（马铃薯、番茄、辣椒）中克隆。 然而，这些基因的分子分析在模式植物如烟草中要容易得多。 这种模型系统的产生需要产生转基因植物的能力，这需要专门的无菌环境，包括植物组织培养室。 后者允许精确控制温度、光照和湿度，并用于转基因材料的再生。 这种材料反过来又用于建立实验系统或验证候选基因和蛋白质参与植物防御。 从这些实验系统中收集的信息可以应用于更好地利用R基因在作物中的天然环境。