Modular microscope with tetrade micromanipulator for functionnal studies of DNA repair activities at chromosomal termini.

带有四重显微操作器的模块化显微镜，用于染色体末端 DNA 修复活性的功能研究。

• 批准号: 389728-2010
• 负责人: Vodenicharov, Momchil
• 金额: $ 1.29万
• 依托单位: Université de Sherbrooke
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=443000
• 关键词: Modular; microscope; tetrade; micromanipulator; functionnal

The termini of linear eukaryotic chromosomes, known as telomeres, resemble DNA double-helix interruptions, the most dangerous among DNA lesions that threaten the integrity of the genome. While chromosomal breaks are immediately subjected to DNA repair, such repair attempts at telomeres can lead to loss of genetic information, genome instability, cellular transformation and, ultimately, cell death. Paradoxically, several proteins involved in sensing and repair of chromosomal breaks reside at telomeres and assist in their maintenance. Our research goal is to define the molecular features that distinguish telomeres from genomic DNA breaks and how DNA repair activities are controlled at telomeres throughout the cell cycle.

线性真核染色体的末端，被称为端粒，类似于DNA双螺旋中断，是威胁基因组完整性的DNA损伤中最危险的。虽然染色体断裂会立即受到DNA修复的影响，但端粒的这种修复尝试会导致遗传信息的丢失、基因组的不稳定、细胞转化，并最终导致细胞死亡。矛盾的是，一些参与感知和修复染色体断裂的蛋白质位于端粒并协助其维持。我们的研究目标是定义区分端粒和基因组DNA断裂的分子特征，以及DNA修复活动在整个细胞周期中如何在端粒中受到控制。