Workstation for quantitative analysis and visualization of multi-dimensional imaging datasets

用于多维成像数据集定量分析和可视化的工作站

• 批准号: 390794-2010
• 负责人: Singh, Jaswant
• 金额: $ 3.54万
• 依托单位: University of Saskatchewan
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=443289
• 关键词: Workstation; quantitative; analysis; visualization; multi

Applicants, Drs. J. Singh, G. Adams and B. Singh, have diverse NSERC-funded research programs that rely heavily on imaging technologies. Dr. J. Singh's research focus is on factors affecting oocyte quality in cattle, Dr. G. Adams is investigating the role of ovulation inducing factor in seminal plasma and early detection of prostatic cancer, and Dr. B. Singh explores the mechanisms of lung inflammation. They study cellular and molecular interactions in healthy and diseased tissues by confocal microscopy, ultrasonography, and synchrotron based x-ray imaging. These imaging methods generate complex and very large datasets representing distribution of cells, sub-cellular structures and functional molecules in tissue volumes. The applicants request funding for a computer workstation to perform quantitative analyses and visualization of these datasets. The workstation will consist of 1) a high-power desktop computer capable of real-time rendering and displaying 3D volumes, 2) a deconvolution software package to correct for optical artifacts introduced by imaging methods, and 3) a segmentation and quantitative software package for separation of voxels representing objects of interest from the rest of tissue. Analysis modules of this software will permit quantitative assessment of differences in structural features (e.g., number, distribution, surface area, volume, spatial inter-relationship) during normal physiologic phases and during disease processes. The applicants are intensely involved in the training of highly-qualified personnel and take pride in celebrating the accomplishments of their students. Collectively, the applicants have trained more than 125 students in their labs since 2003. The availability of the requested equipment will enhance the graduate student experience, improve the quality and impact of their research, and make them more competitive in research careers. We anticipate that several other colleagues in our area will benefit from the imaging workstation.

申请者,Drs。J. Singh， G. Adams和B. Singh，他们有不同的nserc资助的研究项目，这些项目严重依赖于成像技术。J. Singh博士的研究重点是影响牛卵母细胞质量的因素，G. Adams博士正在研究排卵诱导因子在精浆中的作用和前列腺癌的早期检测，B. Singh博士探索肺部炎症的机制。他们通过共聚焦显微镜、超声和基于同步加速器的x射线成像研究健康和病变组织中的细胞和分子相互作用。这些成像方法产生复杂且非常大的数据集，代表细胞、亚细胞结构和功能分子在组织体积中的分布。申请人要求拨款建立计算机工作站，对这些数据集进行定量分析和可视化。该工作站将包括1)一台能够实时渲染和显示3D体积的高功率台式计算机，2)一个反卷积软件包，用于校正由成像方法引入的光学伪影，以及3)一个分割和定量软件包，用于将代表感兴趣对象的体素与其余组织分离。该软件的分析模块将允许在正常生理阶段和疾病过程中对结构特征（例如，数量、分布、表面积、体积、空间相互关系）的差异进行定量评估。申请人积极参与高素质人才的培养，并以庆祝学生的成就而自豪。自2003年以来，这些申请者总共在他们的实验室培训了125多名学生。所需设备的可用性将增强研究生的体验，提高他们的研究质量和影响，并使他们在研究生涯中更具竞争力。我们预计我们地区的其他几位同事将受益于成像工作站。