Dentitrification gene expression in the rhizosophere

根际反硝化基因的表达

• 批准号: 497-2010
• 负责人: Trevors, Jack
• 金额: $ 1.97万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2010
• 资助国家: 加拿大
• 起止时间: 2010-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=449114
• 关键词: Dentitrification; gene; expression; rhizosophere

The overall theme/objective is to understand the link between N2O emissions, denitrification gene expression as determined by reverse transcription-real time PCR, differential total cellular protein expression analyzed via nano-liquid chromatography-MS/MS in pure cultures of Pseudomonas mandelii (soil denitrifier) and the bulk soil and the rhizosphere of wheat plants (proteomics will only be used in pure culture and pure culture in model rhizosphere studies).

总的主题/目标是了解N2O排放、反转录-实时PCR测定的反硝化基因表达、通过纳米液体色谱-MS/MS分析的曼氏假单胞菌(土壤反硝化菌)纯培养物中细胞总蛋白的差异表达与普通土壤和小麦植株根际之间的联系(蛋白质组学将仅在模型根际研究中用于纯培养和纯培养)。