Integrated mass spectrometric methods for the analysis of protein function and proteome regulation

用于分析蛋白质功能和蛋白质组调控的集成质谱方法

• 批准号: 249972-2010
• 负责人: Kast, Juergen
• 金额: $ 2.91万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2010
• 资助国家: 加拿大
• 起止时间: 2010-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=455501
• 关键词: Integrated; mass; spectrometric; methods; analysis

Proteins make up the majority of the content of any type of cell. This family of molecules is also involved in essentially all cellular processes or structures. Understanding how cells work, i.e. how the many different tasks that are being carried out at any point in time are coordinated, requires dedicated analytical tools. Mass spectrometry is a powerful and versatile analytical technique that can be employed for the analysis of individual proteins and modifications they undergo, their interactions, but also of cellular structures, and even entire cells, tissues, and organisms. Our laboratory focuses on the development and implementation of novel mass spectrometric techniques that allow to study cells at all of these different levels. We have a strong track record of innovation, such as our pioneering work on formaldehyde cross-linking for protein interaction studies and our multiplex approaches for protein modification analysis. We have an excellent track record in the training of graduate students in an interdisciplinary environment, which enhances their qualifications and improves their chances for subsequent employment as highly qualified personnel. In this proposal, we seek funding to continue the training of graduate students who will engage in the development and optimization of dedicated mass spectrometry-based and bioinformatic tools for the analysis of individual proteins, their concentration, modification state, stability, interactions, as well as their impact on the regulation of the entire protein content of the cell (the proteome). Several of these methods will be integrated into a more comprehensive workflow, and applied to selected projects in several collaborations, focusing on aspects of mammalian and marine biology.

蛋白质构成任何类型细胞的大部分内容。 这个分子家族也参与基本上所有的细胞过程或结构。 了解细胞如何工作，即在任何时间点执行的许多不同任务如何协调，需要专门的分析工具。 质谱是一种功能强大且用途广泛的分析技术，可用于分析单个蛋白质及其所经历的修饰、它们的相互作用，以及细胞结构，甚至整个细胞、组织和生物体。 我们的实验室专注于开发和实施新的质谱技术，允许在所有这些不同的水平上研究细胞。 我们拥有强大的创新记录，例如我们在蛋白质相互作用研究中的甲醛交联方面的开创性工作以及我们用于蛋白质修饰分析的多重方法。 我们在跨学科环境中培养研究生方面有着良好的记录，这提高了他们的资格，并提高了他们作为高素质人员随后就业的机会。 在这项提案中，我们寻求资金继续培训研究生，他们将参与开发和优化专用的基于质谱和生物信息学的工具，用于分析单个蛋白质，其浓度，修饰状态，稳定性，相互作用以及它们对细胞整个蛋白质含量（蛋白质组）的影响。 其中几种方法将被纳入一个更全面的工作流程，并应用于几个合作项目，重点是哺乳动物和海洋生物学方面。