Understanding the regulation of airway epithelial chloride channels

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The proposed research program is aimed at developing a more in-depth knowledge of the movement of chloride, a component of table salt, in cells lining the entire airway (or windpipe). Proteins called channels allow the movement of chloride out of cells and onto the airway surface. The chloride pulls water with it, thus properly hydrating the airway and protecting it against infection. The program's long-term goal is to discover and define the contribution of channels and related proteins involved in chloride movement in both normal and diseased airway cells. Two short term objectives will be addressed over the next five years regarding the role and action of one specific protein that modulates chloride movement. Initially, the specific gene of the channel that the specific protein acts upon will be identified. Secondly, how the specific protein interacts within the identified channel's structure will be determined, thereby defining a mechanism for a phenomenon that has eluded explanation for over 15 years. Two graduate students will be involved in this research and will receive extensive training in molecular, biochemical, and electrophysiology techniques, skills which are highly desirable and sought after by pharmaceutical industry employers. Their expertise upon graduation will expand their potential range of careers and enhance the reputation of research in Canada. Finally, the knowledge gained through this research will lead to a better understanding of respiratory diseases that impact Canadians, such as cystic fibrosis, asthma, and chronic obstructive pulmonary disease (COPD), and will lead to the development of innovative treatments.