Ussing chamber station for the measurement of current produced by transpithelial ion movement

使用室站测量跨上皮离子运动产生的电流

• 批准号: 390560-2010
• 负责人: Loewen, Matthew
• 金额: $ 2.79万
• 依托单位: University of Saskatchewan
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=443222
• 关键词: Ussing; chamber; station; measurement; current

This equipment request is for an Ussing chamber station. This apparatus will be used by a new faculty member at the University of Saskatchewan for the measurement of current produced by salt movement across cells. The applicant will make extensive use of the equipment in his research program by measuring the movement of chloride, a component of salt, in cells lining the airway (or windpipe). In the long term, his research program will develop a more in-depth knowledge of proteins called channels that allow the movement of chloride out of cells and onto the airway surface. The chloride pulls water with it, thus properly hydrating the airway and protecting it against infection. This research will lead to a better understanding of respiratory diseases that impact Canadians, such as cystic fibrosis, asthma, and chronic obstructive pulmonary disease (COPD), and will lead to the development of innovative treatments. This new equipment is crucial for effective execution of the research program of the applicant. The only equipment available is 25 years old, slow, unreliable, and at times inaccurate. The new equipment will produce results faster because of its larger capacity, be reliable because of its age and quality, give more accurate results because of its new electronics, and run more samples simultaneously.

这个设备要求是一个使用室站。这个仪器将被萨斯喀彻温大学的一位新教员用来测量盐在细胞间运动所产生的电流。申请人将在其研究计划中广泛使用该设备，通过测量气道（或气管）细胞中氯离子（盐的一种成分）的运动。从长远来看，他的研究项目将对一种叫做通道的蛋白质有更深入的了解，这种蛋白质允许氯离子从细胞中移动到气道表面。氯离子会把水拉到气管里，从而适当地给气管补水，保护它不受感染。这项研究将使人们更好地了解影响加拿大人的呼吸系统疾病，如囊性纤维化、哮喘和慢性阻塞性肺疾病（COPD），并将导致创新治疗方法的发展。这种新设备对于申请人的研究计划的有效实施至关重要。唯一可用的设备已经25年了，速度慢，不可靠，有时还不准确。由于容量更大，新设备将更快地得出结果；由于其使用时间和质量，新设备将更可靠；由于其新的电子设备，新设备将给出更准确的结果；