Understanding the regulation of airway epithelial chloride channels

了解气道上皮氯离子通道的调节

• 批准号: 371364-2010
• 负责人: Loewen, Matthew
• 金额: $ 2.33万
• 依托单位: University of Saskatchewan
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=483536
• 关键词: Understanding; regulation; airway; epithelial; chloride

The proposed research program is aimed at developing a more in-depth knowledge of the movement of chloride, a component of table salt, in cells lining the entire airway (or windpipe). Proteins called channels allow the movement of chloride out of cells and onto the airway surface. The chloride pulls water with it, thus properly hydrating the airway and protecting it against infection. The program's long-term goal is to discover and define the contribution of channels and related proteins involved in chloride movement in both normal and diseased airway cells. Two short term objectives will be addressed over the next five years regarding the role and action of one specific protein that modulates chloride movement. Initially, the specific gene of the channel that the specific protein acts upon will be identified. Secondly, how the specific protein interacts within the identified channel's structure will be determined, thereby defining a mechanism for a phenomenon that has eluded explanation for over 15 years. Two graduate students will be involved in this research and will receive extensive training in molecular, biochemical, and electrophysiology techniques, skills which are highly desirable and sought after by pharmaceutical industry employers. Their expertise upon graduation will expand their potential range of careers and enhance the reputation of research in Canada. Finally, the knowledge gained through this research will lead to a better understanding of respiratory diseases that impact Canadians, such as cystic fibrosis, asthma, and chronic obstructive pulmonary disease (COPD), and will lead to the development of innovative treatments.

拟议中的研究计划旨在更深入地了解氯化物（食盐的一种成分）在整个气道（或气管）细胞中的运动。被称为通道的蛋白质允许氯离子从细胞中移动到气道表面。氯化物将水带走，从而适当地水合气道并保护其免受感染。该计划的长期目标是发现和定义参与正常和患病气道细胞中氯离子运动的通道和相关蛋白质的贡献。两个短期目标将在未来五年内解决的作用和行动的一个特定的蛋白质，调节氯离子运动。最初，将鉴定特定蛋白质作用的通道的特定基因。其次，将确定特定蛋白质如何在已识别的通道结构内相互作用，从而确定15年来一直无法解释的现象的机制。两名研究生将参与这项研究，并将接受分子，生物化学和电生理学技术的广泛培训，这些技能是制药行业雇主非常希望和追求的。毕业后他们的专业知识将扩大他们的潜在职业范围，并提高加拿大研究的声誉。最后，通过这项研究获得的知识将有助于更好地了解影响加拿大人的呼吸系统疾病，如囊性纤维化，哮喘和慢性阻塞性肺疾病（COPD），并将导致创新治疗的发展。