Confocal laser scanning microscope for high-resolution, 3-dimensional imaging in neuroscience

用于神经科学中高分辨率 3 维成像的共焦激光扫描显微镜

• 批准号: 406103-2011
• 负责人: Carbonetto, Salvatore
• 金额: $ 10.16万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2010
• 资助国家: 加拿大
• 起止时间: 2010-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=472388
• 关键词: Confocal; laser; scanning; microscope; resolution

This grant will upgrade an existing epifluorescence microscope to a confocal Laser Scanning Microscope (LSM) by purchase of key additional components. The team of 7 applicants led by Salvatore Carbonetto includes researchers at the McGill Centre for Research in Neuroscience. A major focus of our research is to understand how nervous systems function at a cellular and molecular level, and how neural circuits are assembled and remodeled. High-resolution, 3-dimensional microscopy is essential for these studies, but our existing equipment is insufficient because 1) it is too heavily used, 2) it is highly specialized for particular applications, and 3) it lacks specific technical capabilities that we urgently want to exploit in our research programs. We favor the FV1000 components from Olympus because they can be perfectly integrated with an existing Olympus BX61 microscope that was purchased recently. The integrated FV1000-BX61 confocal LSM system will complement our existing infrastructure and provide additional technical capabilities that will permit experiments that are presently unfeasible. This RTI grant provides a unique opportunity for the applicants to acquire this state-of-the-art confocal LSM system, which will help our trainees to 1) complete and publish their research, 2) explore frontiers in experimental neuroscience, and 3) prepare for careers in academic science, the biotech industry, or other sectors.

这笔赠款将通过购买关键的附加部件，将现有的荧光显微镜升级为共焦激光扫描显微镜(LSM)。由塞尔瓦托·卡博内托领导的7名申请者团队包括麦吉尔神经科学研究中心的研究人员。我们研究的一个主要重点是了解神经系统在细胞和分子水平上的功能，以及神经电路是如何组装和重塑的。高分辨率的三维显微镜对于这些研究是必不可少的，但我们现有的设备是不够的，因为1)它的使用量太大，2)它对于特定的应用高度专业化，3)它缺乏我们迫切想要在我们的研究计划中开发的特定技术能力。我们青睐奥林巴斯的FV1000组件，因为它们可以与最近购买的现有奥林巴斯BX61显微镜完美集成。集成的FV1000-BX61共焦LSM系统将补充我们现有的基础设施，并提供额外的技术能力，使目前不可行的实验成为可能。这项RTI资助为申请者提供了一个独特的机会来获得这一最先进的共焦LSM系统，这将帮助我们的学员1)完成并发表他们的研究，2)探索实验神经科学的前沿，3)为学术科学、生物技术行业或其他部门的职业生涯做准备。