Large capacity suspension cell culture CO2 incubator system

大容量悬浮细胞培养CO2培养箱系统

• 批准号: 406788-2011
• 负责人: Ausió, Juan
• 金额: $ 1.73万
• 依托单位: University of Victoria
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2010
• 资助国家: 加拿大
• 起止时间: 2010-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=472558
• 关键词: Large; capacity; suspension; cell; culture

A eukaryotic cell CO2 incubator is a piece of equipment that is used to grow eukaryote cells (such as human or mouse cells). The use of cell systems for biochemical research is very practical and in many instances it circumvents the use of animals. Several aspects of the research pursued in my laboratory (currently funded by NSERC) involve the characterization of structural chromatin transitions that are involved in the cell differentiation process (spermatogenesis) that leads to the formation of male sperm cells (spermiogenesis). An important part of this work involves the reconstitution of chromatin templates using chromosome proteins (histones) consisting of different post-translational modifications. These are biologically-relevant chemical modifications (for instance, acetylation, phosphorylation, methylation) undergone by these proteins. A CO2 incubator is critical for growing in liquid suspension cultures the large amounts of cells required for these types of work. Our past research on spermiogenesis started with the analysis of several fundamental aspects of the spermiogenesis process shared by invertebrate and vertebrate organisms. For the last ten years we have used a second hand CO2 incubator that we inherited from a colleague's lab. As the interests of this research have evolved towards mammalian organisms in recent years, the need for access to a good piece of such equipment has become imperative.

真核细胞CO2培养箱是一种用于培养真核细胞（如人类或小鼠细胞）的设备。使用细胞系统进行生物化学研究是非常实用的，在许多情况下，它可以避免使用动物。在我的实验室（目前由NSERC资助）进行的研究的几个方面涉及结构染色质转换的表征，这些结构染色质转换涉及导致男性精子细胞形成的细胞分化过程（精子发生）。这项工作的一个重要部分涉及使用由不同翻译后修饰组成的染色体蛋白（组蛋白）重建染色质模板。这些是这些蛋白质经历的与生物相关的化学修饰（例如，乙酰化、磷酸化、甲基化）。CO2培养箱对于在液体悬浮培养物中生长这些类型工作所需的大量细胞至关重要。我们过去对精子发生的研究始于对无脊椎动物和脊椎动物生物体共有的精子发生过程的几个基本方面的分析。在过去的十年里，我们一直使用从同事实验室继承的二手CO2培养箱。由于近年来这项研究的兴趣已经发展到哺乳动物有机体，因此需要获得一件好的此类设备已变得势在必行。