Large capacity suspension cell culture CO2 incubator system

真核细胞二氧化碳孵化器是一种用于培养真核细胞(如人或鼠细胞)的设备。使用细胞系统进行生化研究是非常实用的，在许多情况下，它绕过了使用动物。我的实验室(目前由NSERC资助)进行的研究的几个方面涉及结构染色质转变的特征，这些结构染色质转变参与细胞分化过程(精子发生)，导致男性精子细胞的形成(精子发生)。这项工作的一个重要部分涉及使用由不同翻译后修饰组成的染色体蛋白(组蛋白)重建染色质模板。这些都是这些蛋白质所经历的与生物相关的化学修饰(例如，乙酰化、磷酸化、甲基化)。二氧化碳培养箱对于在液体悬浮培养中生长至关重要，液体悬浮培养是这些类型工作所需的大量细胞。我们过去对精子发生的研究始于对无脊椎动物和脊椎动物共有的精子发生过程的几个基本方面的分析。在过去的十年里，我们一直使用从同事的实验室继承下来的二手二氧化碳孵化器。近年来，随着这项研究的兴趣逐渐向哺乳动物生物发展，获得一件好的此类设备的需求变得势在必行。

A eukaryotic cell CO2 incubator is a piece of equipment that is used to grow eukaryote cells (such as human or mouse cells). The use of cell systems for biochemical research is very practical and in many instances it circumvents the use of animals. Several aspects of the research pursued in my laboratory (currently funded by NSERC) involve the characterization of structural chromatin transitions that are involved in the cell differentiation process (spermatogenesis) that leads to the formation of male sperm cells (spermiogenesis). An important part of this work involves the reconstitution of chromatin templates using chromosome proteins (histones) consisting of different post-translational modifications. These are biologically-relevant chemical modifications (for instance, acetylation, phosphorylation, methylation) undergone by these proteins. A CO2 incubator is critical for growing in liquid suspension cultures the large amounts of cells required for these types of work. Our past research on spermiogenesis started with the analysis of several fundamental aspects of the spermiogenesis process shared by invertebrate and vertebrate organisms. For the last ten years we have used a second hand CO2 incubator that we inherited from a colleague's lab. As the interests of this research have evolved towards mammalian organisms in recent years, the need for access to a good piece of such equipment has become imperative.